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Literature DB >> 5001592

The isolation of ovomucoid variants differing in carbohydrate composition.

Abstract

Three major and two minor species of ovomucoid were separated by chromatography on sulphoethyl-Sephadex. The predominant sialic acid-free species was further resolved into three fractions by DEAE-cellulose chromatography. Although all species of ovomucoid had closely similar trypsin-inhibiting activity, immunochemical properties and amino acid composition, they differ in carbohydrate composition. Wide variation was observed in the content of galactose, N-acetylglucosamine and sialic acid. Charge heterogeneity was related, in part, to variation in sialic acid content. The implications of variable carbohydrate composition for the structure and function of ovomucoid are discussed.

Entities: Chemical

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Year: 1971 PMID： 5001592 PMCID： PMC1176971 DOI： 10.1042/bj1230399

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

27 in total

The isolation of ovomucoid variants differing in carbohydrate composition.

1. PRE-ALBUMINS OF EMBRYONIC CHICK PLASMA.

2. HETEROGENEITY OF OVOMUCOID.

3. The carbohydrate of ovomucoid.

4. The specificities of chicken ovomucoid and ovoinhibitor.

5. The attachment of carbohydrate in ovomucoid.

6. The separation and quantitative determination of the component sugars of mucoproteins.

7. Investigations on proteins and polymers. X. Composition and fractionation of ovomucoid.

8. Electrophoretic properties of ovomucoid.

9. Physical and chemical studies on glycoproteins. 3. The microheterogeneity of fetuin, a fetal calf serum glycoprotein.

10. Separation and characterization of the ovoinhibitors from chicken egg white.

1. Active fragments obtained by cyanogen bromide cleavage of ovomucoid.

2. Turtle-dove ovomucoid, a glycoprotein proteinase inhibitor with P1-blood-group antigen activity.

3. Cyanogen bromide cleavage of ovomucoid.

4. Biosynthesis of ovomucoid in vitro.

5. Isolation and characterization of a variant of ovomucoid.

6. A structural study of the asparagine-linked oligosaccharide moiety of duck ovomucoid.

7. Location of the carbohydrate groups of ovomucoid.