Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Promoter and enhancer activities of long terminal repeats associated with cellular retrovirus-like (VL30) elements.

Literature DB >> 3456150

Promoter and enhancer activities of long terminal repeats associated with cellular retrovirus-like (VL30) elements.

Abstract

LTR units associated with cellular retrovirus-like elements are abundantly present in chromosomal DNA of animal cells. We have analyzed the promoter and enhancer activities of diverse LTR units associated with different members of the murine retrovirus-like family known as VL30. We report here that the structurally heterogenous VL30 LTRs displayed highly variable promoter/enhancer activities. The most active VL30 LTR (designated VL3) promoted CAT activity to levels six-fold higher than the LTR of the strongly transforming retrovirus, MSV. This VL30 transcription unit, containing a unique U3 region, was further characterized by S1 nuclease mapping. VL3 LTR functioned as an enhancer in CAT constructs containing a SV40 promoter. In addition, a defined U3 segment was shown to augment expression of CAT in an orientation independent manner. VL3 LTR also served as an efficient promoter and enhancer in heterologous monkey cells. These results suggest that certain resident LTRs possess promoter and enhancer capacities greater than those possessed by LTRs of infectious retroviruses.

Entities: Gene Species

Mesh：

Substances：

Year: 1986 PMID： 3456150 PMCID： PMC339455 DOI： 10.1093/nar/14.2.645

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

21 in total

1. Heterogeneity of "virus-like" genes encoding retrovirus-associated 30S RNA and their organization within the mouse genome.

Authors: E Keshet; Y Shaul; J Kaminchik; H Aviv
Journal: Cell Date: 1980-06 Impact factor: 41.582

2. The long terminal repeat of an endogenous intracisternal A-particle gene functions as a promoter when introduced into eucaryotic cells by transfection.

Authors: K K Lueders; J W Fewell; E L Kuff; T Koch
Journal: Mol Cell Biol Date: 1984-10 Impact factor: 4.272

3. SV40-transformed simian cells support the replication of early SV40 mutants.

Authors: Y Gluzman
Journal: Cell Date: 1981-01 Impact factor: 41.582

4. Host-specific activation of transcription by tandem repeats from simian virus 40 and Moloney murine sarcoma virus.

Authors: L A Laimins; G Khoury; C Gorman; B Howard; P Gruss
Journal: Proc Natl Acad Sci U S A Date: 1982-11 Impact factor: 11.205

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

6. Sequences associated with intracisternal A particles are reiterated in the mouse genome.

Authors: K K Lueders; E L Kuff
Journal: Cell Date: 1977-12 Impact factor: 41.582

7. Patterns of genomic distribution and sequence heterogeneity of a murine "retrovirus-like" multigene family.

Authors: E Keshet; A Itin
Journal: J Virol Date: 1982-07 Impact factor: 5.103

8. Tumorigenic transformation of mammalian cells induced by a normal human gene homologous to the oncogene of Harvey murine sarcoma virus.

Authors: E H Chang; M E Furth; E M Scolnick; D R Lowy
Journal: Nature Date: 1982-06-10 Impact factor: 49.962

9. Some structural and antigenic properties of intracisternal A particles occurring in mouse tumors (complement fixation-immunodiffusion-neuroblastoma-plasma-cell tumor).

Authors: E L Kuff; K K Leuders; H L Ozer; N A Wivel
Journal: Proc Natl Acad Sci U S A Date: 1972-01 Impact factor: 11.205

4. An internal ribosomal entry signal in the rat VL30 region of the Harvey murine sarcoma virus leader and its use in dicistronic retroviral vectors.

Authors: C Berlioz; C Torrent; J L Darlix
Journal: J Virol Date: 1995-10 Impact factor: 5.103

5. Independent regulation of mouse VL30 retrotransposon expression in response to serum and oncogenic cell transformation.

Authors: L Eaton; J D Norton
Journal: Nucleic Acids Res Date: 1990-04-25 Impact factor: 16.971

6. Harvey sarcoma virus genome contains no extensive sequences unrelated to those of other retroviruses except ras.

Authors: K F Manly; G R Anderson; D L Stoler
Journal: J Virol Date: 1988-09 Impact factor: 5.103

7. Transient expression of a chloramphenicol acetyltransferase gene following transfection of Physarum polycephalum myxamoebae.

Authors: K McCurrach; L A Glover; N Hardman
Journal: Curr Genet Date: 1988 Impact factor: 3.886

8. Sequence analysis of Mus dunni endogenous virus reveals a hybrid VL30/gibbon ape leukemia virus-like structure and a distinct envelope.

Authors: G Wolgamot; L Bonham; A D Miller
Journal: J Virol Date: 1998-09 Impact factor: 5.103

9. Inducible and cell type-specific expression of VL30 U3 subgroups correlate with their enhancer design.

Authors: M Nilsson; S Bohm
Journal: J Virol Date: 1994-01 Impact factor: 5.103

10. Anoxia-inducible rat VL30 elements and their relationship to ras-containing sarcoma viruses.

Authors: B A Firulli; G R Anderson; D L Stoler; S D Estes
Journal: J Virol Date: 1993-11 Impact factor: 5.103