Literature DB >> 6260373

SV40-transformed simian cells support the replication of early SV40 mutants.

Y Gluzman.   

Abstract

CV-1, an established line of simian cells permissive for lytic growth of SV40, were transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen. Three transformed lines (COS-1, -3, -7) were established and found to contain T antigen; retain complete permissiveness for lytic growth of SV40; support the replication of tsA209 virus at 40 degrees C; and support the replication of pure populations of SV40 mutants with deletions in the early region. One of the lines (COS-1) contains a single integrated copy of the complete early region of SV40 DNA. These cells are possible hosts for the propagation of pure populations of recombinant SV40 viruses.

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Year:  1981        PMID： 6260373     DOI： 10.1016/0092-8674(81)90282-8

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


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