Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Literature DB >> 518835

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

J M Chirgwin, A E Przybyla, R J MacDonald, W J Rutter.

Abstract

Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was isolated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of alpha-amylase messenger ribonucleic acid.

Entities: Chemical Species

Mesh：

Substances：

Year: 1979 PMID： 518835 DOI： 10.1021/bi00591a005

Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162

Keyword Cloud
Cited

2000 in total

1. Molecular basis of altered contractility in experimental colitis: expression of L-type calcium channel.

Authors: I Khan
Journal: Dig Dis Sci Date: 1999-08 Impact factor: 3.199

2. Soldier caste-specific gene expression in the mandibular glands of Hodotermopsis japonica (Isoptera: termopsidae).

Authors: T Miura; A Kamikouchi; M Sawata; H Takeuchi; S Natori; T Kubo; T Matsumoto
Journal: Proc Natl Acad Sci U S A Date: 1999-11-23 Impact factor: 11.205

3. Characterization of the 5' ends for polyadenylated RNAs synthesized during the replication of hepatitis delta virus.

Authors: S Gudima; K Dingle; T T Wu; G Moraleda; J Taylor
Journal: J Virol Date: 1999-08 Impact factor: 5.103

4. Extracellular matrix modulates enterocyte growth via downregulation of c-jun but is independent of p21 and p27 expression.

Authors: S I Wolpert; K M Lally; J Li; J Y Wang; B L Bass
Journal: J Gastrointest Surg Date: 1999 May-Jun Impact factor: 3.452

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

1. Molecular basis of altered contractility in experimental colitis: expression of L-type calcium channel.

2. Soldier caste-specific gene expression in the mandibular glands of Hodotermopsis japonica (Isoptera: termopsidae).

3. Characterization of the 5' ends for polyadenylated RNAs synthesized during the replication of hepatitis delta virus.

4. Extracellular matrix modulates enterocyte growth via downregulation of c-jun but is independent of p21 and p27 expression.

5. MsJ1, an alfalfa DnaJ-like gene, is tissue-specific and transcriptionally regulated during cell cycle.

6. Analysis of the gene family encoding lipases in Candida rugosa by competitive reverse transcription-PCR.

7. Expression of Bcl-2 in inflammatory sites from patients with active Behçet's disease.

8. Mammary gland sympathetic innervation is a major component in type 1 deiodinase regulation.

9. Paramutation alters regulatory control of the maize pl locus.

10. Cloning of beta-1,3-glucanases expressed during Cichorium somatic embryogenesis.