| Literature DB >> 32393904 |
Qiupeng Lin1,2, Yuan Zong1, Chenxiao Xue1,2, Shengxing Wang1, Shuai Jin1,2, Zixu Zhu1,2, Yanpeng Wang1,2, Andrew V Anzalone3,4,5, Aditya Raguram3,4,5, Jordan L Doman3,4,5, David R Liu3,4,5, Caixia Gao6,7.
Abstract
Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.Entities:
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Year: 2020 PMID： 32393904 DOI： 10.1038/s41587-020-0455-x
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908