Literature DB >> 30094448

Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs.

Thirupathi Ravula1, Nathaniel Z Hardin, Jia Bai, Sang-Choul Im, Lucy Waskell, Ayyalusamy Ramamoorthy.   

Abstract

Although there is a growing interest in using polymer lipid-nanodiscs, the polymer charge poses limitations for studies on membrane proteins. Here, we demonstrate the functional reconstitution of a large soluble-domain containing positively-charged ∼57 kDa cytochrome-P450 and negatively-charged ∼16 kDa cytochrome-b5 in lipid-nanodiscs, and the role of the polymer charge for high-resolution studies on membrane proteins.

Entities:  

Year:  2018        PMID： 30094448      PMCID： PMC6107395          DOI： 10.1039/c8cc04184a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  30 in total

1.  Bioinspired, Size-Tunable Self-Assembly of Polymer-Lipid Bilayer Nanodiscs.

Authors:  Thirupathi Ravula; Sudheer Kumar Ramadugu; Giacomo Di Mauro; Ayyalusamy Ramamoorthy
Journal:  Angew Chem Int Ed Engl       Date:  2017-08-10       Impact factor: 15.336

2.  Reconstitution of the Cytb5-CytP450 Complex in Nanodiscs for Structural Studies using NMR Spectroscopy.

Authors:  Meng Zhang; Rui Huang; Rose Ackermann; Sang-Choul Im; Lucy Waskell; Anna Schwendeman; Ayyalusamy Ramamoorthy
Journal:  Angew Chem Int Ed Engl       Date:  2016-02-29       Impact factor: 15.336

3.  Formation of pH-Resistant Monodispersed Polymer-Lipid Nanodiscs.

Authors:  Thirupathi Ravula; Nathaniel Z Hardin; Sudheer Kumar Ramadugu; Sarah J Cox; Ayyalusamy Ramamoorthy
Journal:  Angew Chem Int Ed Engl       Date:  2018-01-08       Impact factor: 15.336

4.  Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+ channel: the power of native nanodiscs.

Authors:  Jonas M Dörr; Martijn C Koorengevel; Marre Schäfer; Alexander V Prokofyev; Stefan Scheidelaar; Elwin A W van der Cruijsen; Timothy R Dafforn; Marc Baldus; J Antoinette Killian
Journal:  Proc Natl Acad Sci U S A       Date:  2014-12-15       Impact factor: 11.205

Review 5.  The cytochromes P450 and b5 and their reductases--promising targets for structural studies by advanced solid-state NMR spectroscopy.

Authors:  Ulrich H N Dürr; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  Biochim Biophys Acta       Date:  2007-08-24

6.  Significantly shorter Fe-S bond in cytochrome P450-I is consistent with greater reactivity relative to chloroperoxidase.

Authors:  Courtney M Krest; Alexey Silakov; Jonathan Rittle; Timothy H Yosca; Elizabeth L Onderko; Julio C Calixto; Michael T Green
Journal:  Nat Chem       Date:  2015-08-03       Impact factor: 24.427

Review 7.  The catalytic function of cytochrome P450 is entwined with its membrane-bound nature.

Authors:  Carlo Barnaba; Katherine Gentry; Nirupama Sumangala; Ayyalusamy Ramamoorthy
Journal:  F1000Res       Date:  2017-05-09

8.  The effectiveness of styrene-maleic acid (SMA) copolymers for solubilisation of integral membrane proteins from SMA-accessible and SMA-resistant membranes.

Authors:  David J K Swainsbury; Stefan Scheidelaar; Nicholas Foster; Rienk van Grondelle; J Antoinette Killian; Michael R Jones
Journal:  Biochim Biophys Acta Biomembr       Date:  2017-07-24       Impact factor: 3.747

9.  A method for detergent-free isolation of membrane proteins in their local lipid environment.

Authors:  Sarah C Lee; Tim J Knowles; Vincent L G Postis; Mohammed Jamshad; Rosemary A Parslow; Yu-Pin Lin; Adrian Goldman; Pooja Sridhar; Michael Overduin; Stephen P Muench; Timothy R Dafforn
Journal:  Nat Protoc       Date:  2016-06-02       Impact factor: 13.491

Review 10.  The styrene-maleic acid copolymer: a versatile tool in membrane research.

Authors:  Jonas M Dörr; Stefan Scheidelaar; Martijn C Koorengevel; Juan J Dominguez; Marre Schäfer; Cornelis A van Walree; J Antoinette Killian
Journal:  Eur Biophys J       Date:  2015-12-06       Impact factor: 1.733
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  14 in total

Review 1.  Solution NMR: A powerful tool for structural and functional studies of membrane proteins in reconstituted environments.

Authors:  Robbins Puthenveetil; Olga Vinogradova
Journal:  J Biol Chem       Date:  2019-09-24       Impact factor: 5.157

2.  Detergent-free isolation of CYP450-reductase's FMN-binding domain in E. coli lipid-nanodiscs using a charge-free polymer.

Authors:  Bankala Krishnarjuna; Thirupathi Ravula; Ayyalusamy Ramamoorthy
Journal:  Chem Commun (Camb)       Date:  2022-04-14       Impact factor: 6.065

3.  Saponins Form Nonionic Lipid Nanodiscs for Protein Structural Studies by Nuclear Magnetic Resonance Spectroscopy.

Authors:  Samuel D McCalpin; Thirupathi Ravula; Ayyalusamy Ramamoorthy
Journal:  J Phys Chem Lett       Date:  2022-02-14       Impact factor: 6.888

4.  Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids.

Authors:  Bankala Krishnarjuna; Thirupathi Ravula; Ayyalusamy Ramamoorthy
Journal:  Chem Commun (Camb)       Date:  2020-05-28       Impact factor: 6.222

Review 5.  Polymer nanodiscs: Advantages and limitations.

Authors:  Thirupathi Ravula; Nathaniel Z Hardin; Ayyalusamy Ramamoorthy
Journal:  Chem Phys Lipids       Date:  2019-01-29       Impact factor: 3.329

6.  Simple Derivatization of RAFT-Synthesized Styrene-Maleic Anhydride Copolymers for Lipid Disk Formulations.

Authors:  Kevin M Burridge; Benjamin D Harding; Indra D Sahu; Madison M Kearns; Rebecca B Stowe; Madison T Dolan; Richard E Edelmann; Carole Dabney-Smith; Richard C Page; Dominik Konkolewicz; Gary A Lorigan
Journal:  Biomacromolecules       Date:  2020-02-27       Impact factor: 6.988

7.  Metal-Chelated Polymer Nanodiscs for NMR Studies.

Authors:  Nathaniel Z Hardin; Vojč Kocman; Giacomo M Di Mauro; Thirupathi Ravula; Ayyalusamy Ramamoorthy
Journal:  Angew Chem Int Ed Engl       Date:  2019-10-17       Impact factor: 15.336

8.  Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing.

Authors:  Bankala Krishnarjuna; Toshio Yamazaki; G M Anantharamaiah; Ayyalusamy Ramamoorthy
Journal:  Chem Commun (Camb)       Date:  2021-04-16       Impact factor: 6.222

9.  Synthesis, Characterization, and Nanodisc Formation of Non-ionic Polymers*.

Authors:  Thirupathi Ravula; Ayyalusamy Ramamoorthy
Journal:  Angew Chem Int Ed Engl       Date:  2021-06-28       Impact factor: 16.823

Review 10.  Detergent-free systems for structural studies of membrane proteins.

Authors:  Youzhong Guo
Journal:  Biochem Soc Trans       Date:  2021-06-30       Impact factor: 5.407
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