Literature DB >> 32462144

Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids.

Bankala Krishnarjuna1, Thirupathi Ravula, Ayyalusamy Ramamoorthy.   

Abstract

Despite their denaturing properties, detergents are used to purify and study membrane proteins. Herein, we demonstrated a polymer-based detergent-free extraction of the membrane protein cytochrome-b5 along with E. coli lipids. Nuclear magnetic resonance experiments revealed the suitability of using nanodiscs for high-resolution studies and revealed the types of native lipids associated with the protein.

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Year:  2020        PMID： 32462144      PMCID： PMC7337974          DOI： 10.1039/d0cc01737j

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  27 in total

1.  High-level expression in Escherichia coli and purification of the membrane-bound form of cytochrome b(5).

Authors:  S B Mulrooney; L Waskell
Journal:  Protein Expr Purif       Date:  2000-06       Impact factor: 1.650

2.  Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer.

Authors:  Timothy J Knowles; Rachael Finka; Corinne Smith; Yu-Pin Lin; Tim Dafforn; Michael Overduin
Journal:  J Am Chem Soc       Date:  2009-06-10       Impact factor: 15.419

3.  A model of the membrane-bound cytochrome b5-cytochrome P450 complex from NMR and mutagenesis data.

Authors:  Shivani Ahuja; Nicole Jahr; Sang-Choul Im; Subramanian Vivekanandan; Nataliya Popovych; Stéphanie V Le Clair; Rui Huang; Ronald Soong; Jiadi Xu; Kazutoshi Yamamoto; Ravi P Nanga; Angela Bridges; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  J Biol Chem       Date:  2013-05-24       Impact factor: 5.157

4.  Proton-detected 2D radio frequency driven recoupling solid-state NMR studies on micelle-associated cytochrome-b(5).

Authors:  Manoj Kumar Pandey; Subramanian Vivekanandan; Kazutoshi Yamamoto; Sangchoul Im; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  J Magn Reson       Date:  2014-03-01       Impact factor: 2.229

5.  Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein.

Authors:  Shenlin Wang; Rachel A Munro; Lichi Shi; Izuru Kawamura; Takashi Okitsu; Akimori Wada; So-Young Kim; Kwang-Hwan Jung; Leonid S Brown; Vladimir Ladizhansky
Journal:  Nat Methods       Date:  2013-09-08       Impact factor: 28.547

6.  Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+ channel: the power of native nanodiscs.

Authors:  Jonas M Dörr; Martijn C Koorengevel; Marre Schäfer; Alexander V Prokofyev; Stefan Scheidelaar; Elwin A W van der Cruijsen; Timothy R Dafforn; Marc Baldus; J Antoinette Killian
Journal:  Proc Natl Acad Sci U S A       Date:  2014-12-15       Impact factor: 11.205

7.  Stable and Functional Rhomboid Proteases in Lipid Nanodiscs by Using Diisobutylene/Maleic Acid Copolymers.

Authors:  Marta Barniol-Xicota; Steven H L Verhelst
Journal:  J Am Chem Soc       Date:  2018-10-26       Impact factor: 15.419

Review 8.  When detergent meets bilayer: birth and coming of age of lipid bicelles.

Authors:  Ulrich H N Dürr; Ronald Soong; Ayyalusamy Ramamoorthy
Journal:  Prog Nucl Magn Reson Spectrosc       Date:  2013-01-23       Impact factor: 9.795

9.  Expression, purification, and functional reconstitution of 19F-labeled cytochrome b5 in peptide nanodiscs for NMR studies.

Authors:  Jia Bai; Jian Wang; Thirupathi Ravula; Sang-Choul Im; G M Anantharamaiah; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  Biochim Biophys Acta Biomembr       Date:  2020-01-15       Impact factor: 3.747

10.  An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles.

Authors:  Stephen C L Hall; Cecilia Tognoloni; Jack Charlton; Éilís C Bragginton; Alice J Rothnie; Pooja Sridhar; Mark Wheatley; Timothy J Knowles; Thomas Arnold; Karen J Edler; Tim R Dafforn
Journal:  Nanoscale       Date:  2018-06-07       Impact factor: 7.790
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  7 in total

1.  Conformational Tuning of Amylin by Charged Styrene-Maleic-Acid Copolymers.

Authors:  Bikash R Sahoo; Christopher L Souders; Takahiro Watanabe-Nakayama; Zhou Deng; Hunter Linton; Saba Suladze; Magdalena I Ivanova; Bernd Reif; Toshio Ando; Christopher J Martyniuk; Ayyalusamy Ramamoorthy
Journal:  J Mol Biol       Date:  2021-12-06       Impact factor: 5.469

2.  Detergent-free isolation of CYP450-reductase's FMN-binding domain in E. coli lipid-nanodiscs using a charge-free polymer.

Authors:  Bankala Krishnarjuna; Thirupathi Ravula; Ayyalusamy Ramamoorthy
Journal:  Chem Commun (Camb)       Date:  2022-04-14       Impact factor: 6.065

3.  Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing.

Authors:  Bankala Krishnarjuna; Toshio Yamazaki; G M Anantharamaiah; Ayyalusamy Ramamoorthy
Journal:  Chem Commun (Camb)       Date:  2021-04-16       Impact factor: 6.222

4.  Functional Assembly of Caenorhabditis elegans Cytochrome b-2 (Cecytb-2) into Phospholipid Bilayer Nanodisc with Enhanced Iron Reductase Activity.

Authors:  Hamed A Abosharaf; Yuki Sakamoto; Aliaa M Radwan; Keisuke Yuzu; Mika Fujimura; Thoria Diab; Tarek M Mohamed; Eri Chatani; Tetsunari Kimura; Motonari Tsubaki
Journal:  Biomolecules       Date:  2021-01-13

Review 5.  Detergent-Free Isolation of Membrane Proteins and Strategies to Study Them in a Near-Native Membrane Environment.

Authors:  Bankala Krishnarjuna; Ayyalusamy Ramamoorthy
Journal:  Biomolecules       Date:  2022-08-04

6.  Solid-State NMR Study to Probe the Effects of Divalent Metal Ions (Ca2+ and Mg2+) on the Magnetic Alignment of Polymer-Based Lipid Nanodiscs.

Authors:  Thirupathi Ravula; Xiaofeng Dai; Ayyalusamy Ramamoorthy
Journal:  Langmuir       Date:  2021-06-15       Impact factor: 4.331

7.  Silicon Nitride-Based Micro-Apertures Coated with Parylene for the Investigation of Pore Proteins Fused in Free-Standing Lipid Bilayers.

Authors:  Tanzir Ahmed; Jayesh Arun Bafna; Roland Hemmler; Karsten Gall; Richard Wagner; Mathias Winterhalter; Michael J Vellekoop; Sander van den Driesche
Journal:  Membranes (Basel)       Date:  2022-03-09
  7 in total

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