| Literature DB >> 30078725 |
Bing Yao1, Yujing Li2, Zhiqin Wang2, Li Chen3, Mickael Poidevin2, Can Zhang4, Li Lin2, Feng Wang2, Han Bao2, Bin Jiao2, Junghwa Lim2, Ying Cheng2, Luoxiu Huang2, Brittany Lynn Phillips5, Tianlei Xu6, Ranhui Duan7, Kenneth H Moberg4, Hao Wu6, Peng Jin8.
Abstract
A ten-eleven translocation (TET) ortholog exists as a DNA N6-methyladenine (6mA) demethylase (DMAD) in Drosophila. However, the molecular roles of 6mA and DMAD remain unexplored. Through genome-wide 6mA and transcriptome profiling in Drosophila brains and neuronal cells, we found that 6mA may epigenetically regulate a group of genes involved in neurodevelopment and neuronal functions. Mechanistically, DMAD interacts with the Trithorax-related complex protein Wds to maintain active transcription by dynamically demethylating intragenic 6mA. Accumulation of 6mA by depleting DMAD coordinates with Polycomb proteins and contributes to transcriptional repression of these genes. Our findings suggest that active 6mA demethylation by DMAD plays essential roles in fly CNS by orchestrating through added epigenetic mechanisms.Entities:
Keywords: 6-methyladenine; Polycomb; epigenetics; neuron
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Year: 2018 PMID： 30078725 PMCID： PMC6136845 DOI： 10.1016/j.molcel.2018.07.005
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970