| Literature DB >> 25936837 |
Ye Fu1,2, Guan-Zheng Luo1,2, Kai Chen1,2, Xin Deng1,2, Miao Yu1,2, Dali Han1,2, Ziyang Hao1,2, Jianzhao Liu1,2, Xingyu Lu1,2, Louis C Dore1,2, Xiaocheng Weng1,2, Quanjiang Ji1,2, Laurens Mets3, Chuan He1,2.
Abstract
N(6)-methyldeoxyadenosine (6mA or m(6)A) is a DNA modification preserved in prokaryotes to eukaryotes. It is widespread in bacteria and functions in DNA mismatch repair, chromosome segregation, and virulence regulation. In contrast, the distribution and function of 6mA in eukaryotes have been unclear. Here, we present a comprehensive analysis of the 6mA landscape in the genome of Chlamydomonas using new sequencing approaches. We identified the 6mA modification in 84% of genes in Chlamydomonas. We found that 6mA mainly locates at ApT dinucleotides around transcription start sites (TSS) with a bimodal distribution and appears to mark active genes. A periodic pattern of 6mA deposition was also observed at base resolution, which is associated with nucleosome distribution near the TSS, suggesting a possible role in nucleosome positioning. The new genome-wide mapping of 6mA and its unique distribution in the Chlamydomonas genome suggest potential regulatory roles of 6mA in gene expression in eukaryotic organisms.Entities:
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Year: 2015 PMID： 25936837 PMCID： PMC4427561 DOI： 10.1016/j.cell.2015.04.010
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582