| Literature DB >> 26816380 |
Benjamin Delatte1, Fei Wang2, Long Vo Ngoc3, Evelyne Collignon1, Elise Bonvin1, Rachel Deplus1, Emilie Calonne1, Bouchra Hassabi1, Pascale Putmans1, Stephan Awe4, Collin Wetzel5, Judith Kreher4, Romuald Soin3, Catherine Creppe1, Patrick A Limbach5, Cyril Gueydan3, Véronique Kruys3, Alexander Brehm4, Svetlana Minakhina2, Matthieu Defrance1, Ruth Steward2, François Fuks6.
Abstract
Hydroxymethylcytosine, well described in DNA, occurs also in RNA. Here, we show that hydroxymethylcytosine preferentially marks polyadenylated RNAs and is deposited by Tet in Drosophila. We map the transcriptome-wide hydroxymethylation landscape, revealing hydroxymethylcytosine in the transcripts of many genes, notably in coding sequences, and identify consensus sites for hydroxymethylation. We found that RNA hydroxymethylation can favor mRNA translation. Tet and hydroxymethylated RNA are found to be most abundant in the Drosophila brain, and Tet-deficient fruitflies suffer impaired brain development, accompanied by decreased RNA hydroxymethylation. This study highlights the distribution, localization, and function of cytosine hydroxymethylation and identifies central roles for this modification in Drosophila.Entities:
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Year: 2016 PMID： 26816380 DOI： 10.1126/science.aac5253
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728