Literature DB >> 29914645

Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins.

Jasmina Radoicic1, Sang Ho Park1, Stanley J Opella2.   

Abstract

Macrodiscs, which are magnetically alignable lipid bilayer discs with diameters of >30 nm, were obtained by solubilizing protein-containing liposomes with styrene-maleic acid copolymers. Macrodiscs provide a detergent-free phospholipid bilayer environment for biophysical and functional studies of membrane proteins under physiological conditions. The narrow resonance linewidths observed from membrane proteins in styrene-maleic acid macrodiscs advance structure determination by oriented sample solid-state NMR spectroscopy.
Copyright © 2018 Biophysical Society. Published by Elsevier Inc. All rights reserved.

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Year:  2018        PMID: 29914645      PMCID: PMC6035293          DOI: 10.1016/j.bpj.2018.05.024

Source DB:  PubMed          Journal:  Biophys J        ISSN: 0006-3495            Impact factor:   4.033


  31 in total

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6.  Tuning the size of styrene-maleic acid copolymer-lipid nanoparticles (SMALPs) using RAFT polymerization for biophysical studies.

Authors:  Andrew F Craig; Emily E Clark; Indra D Sahu; Rongfu Zhang; Nick D Frantz; M Sameer Al-Abdul-Wahid; Carole Dabney-Smith; Dominik Konkolewicz; Gary A Lorigan
Journal:  Biochim Biophys Acta       Date:  2016-08-15

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Review 10.  The styrene-maleic acid copolymer: a versatile tool in membrane research.

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  15 in total

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Review 6.  Polymer nanodiscs: Advantages and limitations.

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7.  Magnetic Alignment of Polymer Nanodiscs Probed by Solid-State NMR Spectroscopy.

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8.  Metal-Chelated Polymer Nanodiscs for NMR Studies.

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9.  A theoretical assessment of structure determination of multi-span membrane proteins by oriented sample solid-state NMR spectroscopy.

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10.  Solid-State NMR Study to Probe the Effects of Divalent Metal Ions (Ca2+ and Mg2+) on the Magnetic Alignment of Polymer-Based Lipid Nanodiscs.

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