| Literature DB >> 27732795 |
Abstract
Double-strand breaks (DSBs) pose a severe challenge to genome integrity; consequently, cells have developed efficient mechanisms to repair DSBs through several pathways of homologous recombination and other nonhomologous end-joining processes. Much of our understanding of these pathways has come from the analysis of site-specific DSBs created by the HO endonuclease in the budding yeast Saccharomyces cerevisiae. I was fortunate to get in on the ground floor of analyzing the fate of synchronously induced DSBs through the study of what I coined "in vivo biochemistry." I have had the remarkable good fortune to profit from the development of new techniques that have permitted an ever more detailed dissection of these repair mechanisms, which are described here.Entities:
Keywords: DNA damage checkpoint; DNA repair; autobiography; budding yeast mating type–switching; homologous recombination; nonhomologous end-joining; site-specific endonuclease
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Year: 2016 PMID： 27732795 DOI： 10.1146/annurev-genet-120215-035043
Source DB: PubMed Journal: Annu Rev Genet ISSN： 0066-4197 Impact factor: 16.830