| Literature DB >> 30652105 |
Hannah L Klein1, Giedrė Bačinskaja2, Jun Che3, Anais Cheblal4, Rajula Elango5, Anastasiya Epshtein1, Devon M Fitzgerald6,7,8,9, Belén Gómez-González10, Sharik R Khan11, Sandeep Kumar7, Bryan A Leland12, Léa Marie13, Qian Mei14, Judith Miné-Hattab15,16, Alicja Piotrowska17, Erica J Polleys18, Christopher D Putnam19,20, Elina A Radchenko18, Anissia Ait Saada21,22, Cynthia J Sakofsky23, Eun Yong Shim3, Mathew Stracy24, Jun Xia6,7,8,9, Zhenxin Yan7, Yi Yin25, Andrés Aguilera10, Juan Lucas Argueso26, Catherine H Freudenreich18,27, Susan M Gasser4, Dmitry A Gordenin23, James E Haber28, Grzegorz Ira7, Sue Jinks-Robertson29, Megan C King12, Richard D Kolodner19,30,31,32, Andrei Kuzminov11, Sarah Ae Lambert21,22, Sang Eun Lee3, Kyle M Miller6,33, Sergei M Mirkin18, Thomas D Petes25, Susan M Rosenberg6,7,8,9,14, Rodney Rothstein34, Lorraine S Symington13, Pawel Zawadzki17, Nayun Kim35, Michael Lisby2, Anna Malkova5.
Abstract
Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.Keywords: DNA breaks; DNA repair centers; DNA resection; DSBs; Holliday junctions; R-loops; chromatin dynamics; chromosome rearrangements; crossovers; fluorescent proteins; gene amplification; gene conversion; genome instability; gross chromosome rearrangements; homologous recombination; mitotic recombination; mutagenesis; pulsed field gel electrophoresis; replication fork stalling; single-particle tracking; sister chromatid recombination; sister repetitive sequences; site-specific chromosome breaks; toxic recombination intermediates; yeast artificial chromosome
Year: 2019 PMID： 30652105 PMCID： PMC6334234 DOI： 10.15698/mic2019.01.664
Source DB: PubMed Journal: Microb Cell ISSN： 2311-2638