Literature DB >> 32840801

High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products.

Dionna Gamble1,2, Yee Fang Hum3, Sue Jinks-Robertson4.   

Abstract

Mitotic double-strand breaks (DSBs) are repaired by recombination with a homologous donor duplex. This process involves the exchange of single DNA strands between the broken molecule and the repair template, giving rise to regions of heteroduplex DNA (hetDNA). The creation of a defined DSB coupled with the use of a sequence-diverged repair template allows the fine-structure mapping of hetDNA through the sequencing of recombination products. A high-throughput method is described that capitalizes on the single-molecule real-time (SMRT) sequencing technology developed by PacBio. This method allows simultaneous analysis of the hetDNA contained within hundreds of recombination products.

Keywords:  Double-strand break; Gene conversion; PacBio; Recombination; SMRT; Yeast; hetDNA

Mesh：

Substances：

Year:  2021        PMID： 32840801      PMCID： PMC8335798          DOI： 10.1007/978-1-0716-0644-5_34

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

Review 1.  A Life Investigating Pathways That Repair Broken Chromosomes.

Authors:  James E Haber
Journal:  Annu Rev Genet       Date:  2016-10-03       Impact factor: 16.830

2.  Mechanistic View and Genetic Control of DNA Recombination during Meiosis.

Authors:  Marie-Claude Marsolier-Kergoat; Md Muntaz Khan; Jonathan Schott; Xuan Zhu; Bertrand Llorente
Journal:  Mol Cell       Date:  2018-04-05       Impact factor: 17.970

3.  Regulation of hetDNA Length during Mitotic Double-Strand Break Repair in Yeast.

Authors:  Xiaoge Guo; Yee Fang Hum; Kevin Lehner; Sue Jinks-Robertson
Journal:  Mol Cell       Date:  2017-08-03       Impact factor: 17.970

4.  Molecular structures of crossover and noncrossover intermediates during gap repair in yeast: implications for recombination.

Authors:  Katrina Mitchel; Hengshan Zhang; Caroline Welz-Voegele; Sue Jinks-Robertson
Journal:  Mol Cell       Date:  2010-04-23       Impact factor: 17.970

5.  Genome-wide analysis of heteroduplex DNA in mismatch repair-deficient yeast cells reveals novel properties of meiotic recombination pathways.

Authors:  Emmanuelle Martini; Valérie Borde; Matthieu Legendre; Stéphane Audic; Béatrice Regnault; Guillaume Soubigou; Bernard Dujon; Bertrand Llorente
Journal:  PLoS Genet       Date:  2011-09-29       Impact factor: 5.917

Review 6.  Mechanisms and regulation of mitotic recombination in Saccharomyces cerevisiae.

Authors:  Lorraine S Symington; Rodney Rothstein; Michael Lisby
Journal:  Genetics       Date:  2014-11       Impact factor: 4.562

7.  High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast.

Authors:  Yi Yin; Margaret Dominska; Eunice Yim; Thomas D Petes
Journal:  Elife       Date:  2017-07-17       Impact factor: 8.140

8.  Mismatch recognition and subsequent processing have distinct effects on mitotic recombination intermediates and outcomes in yeast.

Authors:  Yee Fang Hum; Sue Jinks-Robertson
Journal:  Nucleic Acids Res       Date:  2019-05-21       Impact factor: 16.971

9.  Heteroduplex DNA position defines the roles of the Sgs1, Srs2, and Mph1 helicases in promoting distinct recombination outcomes.

Authors:  Katrina Mitchel; Kevin Lehner; Sue Jinks-Robertson
Journal:  PLoS Genet       Date:  2013-03-14       Impact factor: 5.917

10.  SMRT Sequencing for Parallel Analysis of Multiple Targets and Accurate SNP Phasing.

Authors:  Xiaoge Guo; Kevin Lehner; Karen O'Connell; Jenny Zhang; Sandeep S Dave; Sue Jinks-Robertson
Journal:  G3 (Bethesda)       Date:  2015-10-23       Impact factor: 3.154
  10 in total

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