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Literature DB >> 24203099

High-dimensionality 13C direct-detected NMR experiments for the automatic assignment of intrinsically disordered proteins.

Wolfgang Bermel¹, Isabella C Felli, Leonardo Gonnelli, Wiktor Koźmiński, Alessandro Piai, Roberta Pierattelli, Anna Zawadzka-Kazimierczuk.

Abstract

We present three novel exclusively heteronuclear 5D (13)C direct-detected NMR experiments, namely (H(N-flip)N)CONCACON, (HCA)CONCACON and (H)CACON(CA)CON, designed for easy sequence-specific resonance assignment of intrinsically disordered proteins (IDPs). The experiments proposed have been optimized to overcome the drawbacks which may dramatically complicate the characterization of IDPs by NMR, namely the small dispersion of chemical shifts and the fast exchange of the amide protons with the solvent. A fast and reliable automatic assignment of α-synuclein chemical shifts was obtained with the Tool for SMFT-based Assignment of Resonances (TSAR) program based on the information provided by these experiments.

Entities: Chemical Disease Gene

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Year: 2013 PMID： 24203099 DOI： 10.1007/s10858-013-9793-z

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

43 in total

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Authors: H J Dyson; P E Wright
Journal: Methods Enzymol Date: 2001 Impact factor: 1.600

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Authors: P E Wright; H J Dyson
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Review 3. Random sampling in multidimensional NMR spectroscopy.

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Review 4. Atomic-level characterization of disordered protein ensembles.

Authors: Tanja Mittag; Julie D Forman-Kay
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5. 4D non-uniformly sampled HCBCACON and ¹J(NCα)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins.

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7. Direct correlation of consecutive C'-N groups in proteins: a method for the assignment of intrinsically disordered proteins.

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8. Rapid characterization of hydrogen exchange in proteins.

Authors: Anushikha Thakur; Kousik Chandra; Abhinav Dubey; Patrick D'Silva; Hanudatta S Atreya
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9. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors: F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
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10. A new method for purification of recombinant human alpha-synuclein in Escherichia coli.

Authors: Chunjuan Huang; Guoping Ren; Hui Zhou; Chih-chen Wang
Journal: Protein Expr Purif Date: 2005-03-30 Impact factor: 1.650

21 in total

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2. Direct ¹³C-detected NMR experiments for mapping and characterization of hydrogen bonds in RNA.

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3. A reduced dimensionality NMR pulse sequence and an efficient protocol for unambiguous assignment in intrinsically disordered proteins.

Authors: Jithender G Reddy; Ramakrishna V Hosur
Journal: J Biomol NMR Date: 2014-05-23 Impact factor: 2.835

4. A six-dimensional alpha proton detection-based APSY experiment for backbone assignment of intrinsically disordered proteins.

Authors: Xuejun Yao; Stefan Becker; Markus Zweckstetter
Journal: J Biomol NMR Date: 2014-11-04 Impact factor: 2.835

5. ¹⁵N detection harnesses the slow relaxation property of nitrogen: Delivering enhanced resolution for intrinsically disordered proteins.

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Journal: Proc Natl Acad Sci U S A Date: 2018-02-05 Impact factor: 11.205