Literature DB >> 22289053

A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum.

Yane-Shih Wang1, Xinqiang Fang, Ashley L Wallace, Bo Wu, Wenshe R Liu.   

Abstract

Together with tRNA(CUA)(Pyl), a rationally designed pyrrolysyl-tRNA synthetase mutant N346A/C348A has been successfully used for the genetic incorporation of a variety of phenylalanine derivatives with large para substituents into superfolder green fluorescent protein at an amber mutation site in Escherichia coli. This discovery greatly expands the genetically encoded noncanonical amino acid inventory and opens the gate for the genetic incorporation of other phenylalanine derivatives using engineered pyrrolysyl-tRNA synthetase-tRNA(CUA)(Pyl) pairs.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 22289053      PMCID： PMC3288562          DOI： 10.1021/ja211972x

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  33 in total

Review 1.  Expanding the genetic code.

Authors:  Lei Wang; Jianming Xie; Peter G Schultz
Journal:  Annu Rev Biophys Biomol Struct       Date:  2006

2.  A facile system for encoding unnatural amino acids in mammalian cells.

Authors:  Peng R Chen; Dan Groff; Jiantao Guo; Weijia Ou; Susan Cellitti; Bernhard H Geierstanger; Peter G Schultz
Journal:  Angew Chem Int Ed Engl       Date:  2009       Impact factor: 15.336

3.  The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of L-phenylalanine and its derivatives.

Authors:  Yane-Shih Wang; William K Russell; Zhiyong Wang; Wei Wan; Lindsey E Dodd; Pei-Jing Pai; David H Russell; Wenshe R Liu
Journal:  Mol Biosyst       Date:  2011-01-14

4.  Expanding the genetic code of Escherichia coli.

Authors:  L Wang; A Brock; B Herberich; P G Schultz
Journal:  Science       Date:  2001-04-20       Impact factor: 47.728

5.  Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine.

Authors:  Deqiang Zhang; Nagarajan Vaidehi; William A Goddard; Joseph F Danzer; Derek Debe
Journal:  Proc Natl Acad Sci U S A       Date:  2002-05-14       Impact factor: 11.205

6.  Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA.

Authors:  Gayathri Srinivasan; Carey M James; Joseph A Krzycki
Journal:  Science       Date:  2002-05-24       Impact factor: 47.728

7.  One plasmid selection system for the rapid evolution of aminoacyl-tRNA synthetases.

Authors:  Charles E Melançon; Peter G Schultz
Journal:  Bioorg Med Chem Lett       Date:  2009-04-09       Impact factor: 2.823

8.  Genetically encoded photocontrol of protein localization in mammalian cells.

Authors:  Arnaud Gautier; Duy P Nguyen; Hrvoje Lusic; Wenlin An; Alexander Deiters; Jason W Chin
Journal:  J Am Chem Soc       Date:  2010-03-31       Impact factor: 15.419

9.  Expanding the genetic code of an animal.

Authors:  Sebastian Greiss; Jason W Chin
Journal:  J Am Chem Soc       Date:  2011-08-22       Impact factor: 15.419

10.  Expanding the genetic code of yeast for incorporation of diverse unnatural amino acids via a pyrrolysyl-tRNA synthetase/tRNA pair.

Authors:  Susan M Hancock; Rajendra Uprety; Alexander Deiters; Jason W Chin
Journal:  J Am Chem Soc       Date:  2010-10-27       Impact factor: 15.419
View more
  65 in total

1.  Pyrrolysyl-tRNA synthetase variants reveal ancestral aminoacylation function.

Authors:  Jae-hyeong Ko; Yane-Shih Wang; Akiyoshi Nakamura; Li-Tao Guo; Dieter Söll; Takuya Umehara
Journal:  FEBS Lett       Date:  2013-08-28       Impact factor: 4.124

2.  Facile Removal of Leader Peptides from Lanthipeptides by Incorporation of a Hydroxy Acid.

Authors:  Noah A Bindman; Silvia C Bobeica; Wenshe R Liu; Wilfred A van der Donk
Journal:  J Am Chem Soc       Date:  2015-06-01       Impact factor: 15.419

3.  A genetically encoded aldehyde for rapid protein labelling.

Authors:  Alfred Tuley; Yan-Jiun Lee; Bo Wu; Zhiyong U Wang; Wenshe R Liu
Journal:  Chem Commun (Camb)       Date:  2014-07-18       Impact factor: 6.222

4.  Genetic Encoding of Three Distinct Noncanonical Amino Acids Using Reprogrammed Initiator and Nonsense Codons.

Authors:  Jeffery M Tharp; Oscar Vargas-Rodriguez; Alanna Schepartz; Dieter Söll
Journal:  ACS Chem Biol       Date:  2021-03-16       Impact factor: 5.100

Review 5.  Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool.

Authors:  Wei Wan; Jeffery M Tharp; Wenshe R Liu
Journal:  Biochim Biophys Acta       Date:  2014-03-12

6.  Expanding the library and substrate diversity of the pyrrolysyl-tRNA synthetase to incorporate unnatural amino acids containing conjugated rings.

Authors:  Vanessa K Lacey; Gordon V Louie; Joseph P Noel; Lei Wang
Journal:  Chembiochem       Date:  2013-09-09       Impact factor: 3.164

7.  Gleaning unexpected fruits from hard-won synthetases: probing principles of permissivity in non-canonical amino acid-tRNA synthetases.

Authors:  Richard B Cooley; P Andrew Karplus; Ryan A Mehl
Journal:  Chembiochem       Date:  2014-07-11       Impact factor: 3.164

8.  Reversible and Tunable Photoswitching of Protein Function through Genetic Encoding of Azobenzene Amino Acids in Mammalian Cells.

Authors:  Ji Luo; Subhas Samanta; Marino Convertino; Nikolay V Dokholyan; Alexander Deiters
Journal:  Chembiochem       Date:  2018-10-02       Impact factor: 3.164

9.  Upgrading protein synthesis for synthetic biology.

Authors:  Patrick O'Donoghue; Jiqiang Ling; Yane-Shih Wang; Dieter Söll
Journal:  Nat Chem Biol       Date:  2013-10       Impact factor: 15.040

10.  Discrimination of colon cancer stem cells using noncanonical amino acid.

Authors:  Xinrui Duan; Honglin Li; Hexin Chen; Qian Wang
Journal:  Chem Commun (Camb)       Date:  2012-07-30       Impact factor: 6.222
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.