Literature DB >> 24019075

Expanding the library and substrate diversity of the pyrrolysyl-tRNA synthetase to incorporate unnatural amino acids containing conjugated rings.

Vanessa K Lacey1, Gordon V Louie, Joseph P Noel, Lei Wang.   

Abstract

Unnatural amino acids (UAAs) containing conjugated ring systems are of interest for their optical properties. Until now, such bulky and planar UAAs could not be incorporated into proteins using the pyrrolysyl tRNA/synthetase shuttling system. Using the "small-intelligent" approach to construct a highly diverse library, we evolved novel synthetases specific for two such UAAs and incorporated them into proteins in E. coli and mammalian cells.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  directed evolution; genetic code expansion; pyrrolysine; small-intelligent library; unnatural amino acids

Mesh：

Substances：

Year:  2013        PMID： 24019075      PMCID： PMC3947478          DOI： 10.1002/cbic.201300400

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  25 in total

1.  Coot: model-building tools for molecular graphics.

Authors:  Paul Emsley; Kevin Cowtan
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-11-26

2.  Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space.

Authors:  Manfred T Reetz; Li-Wen Wang; Marco Bocola
Journal:  Angew Chem Int Ed Engl       Date:  2006-02-13       Impact factor: 15.336

Review 3.  Expanding the genetic code.

Authors:  Lei Wang; Peter G Schultz
Journal:  Angew Chem Int Ed Engl       Date:  2004-12-17       Impact factor: 15.336

4.  Crystallographic studies on multiple conformational states of active-site loops in pyrrolysyl-tRNA synthetase.

Authors:  Tatsuo Yanagisawa; Ryohei Ishii; Ryuya Fukunaga; Takatsugu Kobayashi; Kensaku Sakamoto; Shigeyuki Yokoyama
Journal:  J Mol Biol       Date:  2008-02-29       Impact factor: 5.469

5.  Structure of pyrrolysyl-tRNA synthetase, an archaeal enzyme for genetic code innovation.

Authors:  Jennifer M Kavran; Sarath Gundllapalli; Patrick O'Donoghue; Markus Englert; Dieter Söll; Thomas A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  2007-06-25       Impact factor: 11.205

6.  Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases.

Authors:  Takahito Mukai; Takatsugu Kobayashi; Nobumasa Hino; Tatsuo Yanagisawa; Kensaku Sakamoto; Shigeyuki Yokoyama
Journal:  Biochem Biophys Res Commun       Date:  2008-05-08       Impact factor: 3.575

Review 7.  Expanding the genetic code for biological studies.

Authors:  Qian Wang; Angela R Parrish; Lei Wang
Journal:  Chem Biol       Date:  2009-03-27

8.  Pyrrolysine is not hardwired for cotranslational insertion at UAG codons.

Authors:  Alexandre Ambrogelly; Sarath Gundllapalli; Stephanie Herring; Carla Polycarpo; Carina Frauer; Dieter Söll
Journal:  Proc Natl Acad Sci U S A       Date:  2007-02-20       Impact factor: 11.205

9.  Multistep engineering of pyrrolysyl-tRNA synthetase to genetically encode N(epsilon)-(o-azidobenzyloxycarbonyl) lysine for site-specific protein modification.

Authors:  Tatsuo Yanagisawa; Ryohei Ishii; Ryuya Fukunaga; Takatsugu Kobayashi; Kensaku Sakamoto; Shigeyuki Yokoyama
Journal:  Chem Biol       Date:  2008-11-24

10.  Genetically encoding unnatural amino acids for cellular and neuronal studies.

Authors:  Wenyuan Wang; Jeffrey K Takimoto; Gordon V Louie; Thomas J Baiga; Joseph P Noel; Kuo-Fen Lee; Paul A Slesinger; Lei Wang
Journal:  Nat Neurosci       Date:  2007-07-01       Impact factor: 24.884
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  21 in total

1.  Engineered Aminoacyl-tRNA Synthetases with Improved Selectivity toward Noncanonical Amino Acids.

Authors:  Hui Si Kwok; Oscar Vargas-Rodriguez; Sergey V Melnikov; Dieter Söll
Journal:  ACS Chem Biol       Date:  2019-04-09       Impact factor: 5.100

Review 2.  Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool.

Authors:  Wei Wan; Jeffery M Tharp; Wenshe R Liu
Journal:  Biochim Biophys Acta       Date:  2014-03-12

3.  Genetically Encoding Quinoline Reverses Chromophore Charge and Enables Fluorescent Protein Brightening in Acidic Vesicles.

Authors:  Caiyun Fu; Tomonori Kobayashi; Nanxi Wang; Christian Hoppmann; Bing Yang; Roshanak Irannejad; Lei Wang
Journal:  J Am Chem Soc       Date:  2018-08-22       Impact factor: 15.419

4.  Proximity-enabled protein crosslinking through genetically encoding haloalkane unnatural amino acids.

Authors:  Zheng Xiang; Vanessa K Lacey; Haiyan Ren; Jing Xu; David J Burban; Patricia A Jennings; Lei Wang
Journal:  Angew Chem Int Ed Engl       Date:  2014-01-21       Impact factor: 15.336

5.  Genetically encoding photoswitchable click amino acids in Escherichia coli and mammalian cells.

Authors:  Christian Hoppmann; Vanessa K Lacey; Gordon V Louie; Jing Wei; Joseph P Noel; Lei Wang
Journal:  Angew Chem Int Ed Engl       Date:  2014-03-11       Impact factor: 15.336

6.  Genetically encoding thyronine for fluorescent detection of peroxynitrite.

Authors:  Shanshan Li; Bing Yang; Tomonori Kobayashi; Bingchen Yu; Jun Liu; Lei Wang
Journal:  Bioorg Med Chem       Date:  2020-07-29       Impact factor: 3.641

7.  Genetically encoded releasable photo-cross-linking strategies for studying protein-protein interactions in living cells.

Authors:  Yi Yang; Haiping Song; Dan He; Shuai Zhang; Shizhong Dai; Xiao Xie; Shixian Lin; Ziyang Hao; Huangtao Zheng; Peng R Chen
Journal:  Nat Protoc       Date:  2017-09-21       Impact factor: 13.491

8.  Using Protein-Confined Proximity To Determine Chemical Reactivity.

Authors:  Tomonori Kobayashi; Christian Hoppmann; Bing Yang; Lei Wang
Journal:  J Am Chem Soc       Date:  2016-11-04       Impact factor: 15.419

9.  Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles.

Authors:  Nanxi Wang; Lei Wang
Journal:  Methods Enzymol       Date:  2020-04-25       Impact factor: 1.600

10.  Genetically Encoding Fluorosulfate-l-tyrosine To React with Lysine, Histidine, and Tyrosine via SuFEx in Proteins in Vivo.

Authors:  Nanxi Wang; Bing Yang; Caiyun Fu; He Zhu; Feng Zheng; Tomonori Kobayashi; Jun Liu; Shanshan Li; Cheng Ma; Peng G Wang; Qian Wang; Lei Wang
Journal:  J Am Chem Soc       Date:  2018-04-05       Impact factor: 15.419
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