| Literature DB >> 24756176 |
Alfred Tuley1, Yan-Jiun Lee, Bo Wu, Zhiyong U Wang, Wenshe R Liu.
Abstract
Using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.Entities:
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Year: 2014 PMID: 24756176 PMCID: PMC4062584 DOI: 10.1039/c4cc02000f
Source DB: PubMed Journal: Chem Commun (Camb) ISSN: 1359-7345 Impact factor: 6.222