Literature DB >> 19378306

A facile system for encoding unnatural amino acids in mammalian cells.

Peng R Chen1, Dan Groff, Jiantao Guo, Weijia Ou, Susan Cellitti, Bernhard H Geierstanger, Peter G Schultz.   

Abstract

A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl-tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl-tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA-aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins.

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Year:  2009        PMID： 19378306      PMCID： PMC2873846          DOI： 10.1002/anie.200900683

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  21 in total

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5.  A chemical toolkit for proteins--an expanded genetic code.

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6.  Expanding the genetic code of Escherichia coli.

Authors:  L Wang; A Brock; B Herberich; P G Schultz
Journal:  Science       Date:  2001-04-20       Impact factor: 47.728

Review 7.  The direct genetic encoding of pyrrolysine.

Authors:  Joseph A Krzycki
Journal:  Curr Opin Microbiol       Date:  2005-10-26       Impact factor: 7.934

8.  A genetically encoded photocaged amino acid.

Authors:  Ning Wu; Alexander Deiters; T Ashton Cropp; David King; Peter G Schultz
Journal:  J Am Chem Soc       Date:  2004-11-10       Impact factor: 15.419

9.  Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA.

Authors:  Gayathri Srinivasan; Carey M James; Joseph A Krzycki
Journal:  Science       Date:  2002-05-24       Impact factor: 47.728

10.  Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.

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Journal:  Nature       Date:  2004-08-25       Impact factor: 49.962
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  90 in total

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6.  Light-induced protein translocation by genetically encoded unnatural amino acid in Caenorhabditis elegans.

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Review 8.  Incorporation of Non-Canonical Amino Acids.

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9.  A genetically encoded cyclobutene probe for labelling of live cells.

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10.  Molecular reconstruction of a fungal genetic code alteration.

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