Literature DB >> 19398201

One plasmid selection system for the rapid evolution of aminoacyl-tRNA synthetases.

Charles E Melançon1, Peter G Schultz.   

Abstract

We have developed a rapid, straightforward, one plasmid dual positive/negative selection system for the evolution of aminoacyl-tRNA synthetases with altered specificities in Escherichia coli. This system utilizes an amber stop codon containing chloramphenicol acetyltransferase/uracil phosphoribosyltransferase fusion gene. We demonstrate the utility of the system by identifying a variant of the Methanococcus jannaschii tyrosyl synthetase from a library of 10(9) variants that selectively incorporates para-iodophenylalanine in response to an amber stop codon.

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Year:  2009        PMID： 19398201      PMCID： PMC2714362          DOI： 10.1016/j.bmcl.2009.04.007

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  10 in total

1.  A network of orthogonal ribosome x mRNA pairs.

Authors:  Oliver Rackham; Jason W Chin
Journal:  Nat Chem Biol       Date:  2005-07-17       Impact factor: 15.040

2.  A chemical toolkit for proteins--an expanded genetic code.

Authors:  Jianming Xie; Peter G Schultz
Journal:  Nat Rev Mol Cell Biol       Date:  2006-08-23       Impact factor: 94.444

3.  Addition of an alpha-hydroxy acid to the genetic code of bacteria.

Authors:  Jiantao Guo; Jiangyun Wang; J Christopher Anderson; Peter G Schultz
Journal:  Angew Chem Int Ed Engl       Date:  2008       Impact factor: 15.336

4.  A genetically encoded bidentate, metal-binding amino acid.

Authors:  Jianming Xie; Wenshe Liu; Peter G Schultz
Journal:  Angew Chem Int Ed Engl       Date:  2007       Impact factor: 15.336

5.  A genetically encoded boronate-containing amino acid.

Authors:  Eric Brustad; Mark L Bushey; Jae Wook Lee; Dan Groff; Wenshe Liu; Peter G Schultz
Journal:  Angew Chem Int Ed Engl       Date:  2008       Impact factor: 15.336

6.  Recombinant expression of selectively sulfated proteins in Escherichia coli.

Authors:  Chang C Liu; Peter G Schultz
Journal:  Nat Biotechnol       Date:  2006-10-29       Impact factor: 54.908

7.  In vivo incorporation of unnatural amino acids to probe structure, dynamics, and ligand binding in a large protein by nuclear magnetic resonance spectroscopy.

Authors:  Susan E Cellitti; David H Jones; Leanna Lagpacan; Xueshi Hao; Qiong Zhang; Huiyong Hu; Scott M Brittain; Achim Brinker; Jeremy Caldwell; Badry Bursulaya; Glen Spraggon; Ansgar Brock; Youngha Ryu; Tetsuo Uno; Peter G Schultz; Bernhard H Geierstanger
Journal:  J Am Chem Soc       Date:  2008-06-25       Impact factor: 15.419

8.  The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination.

Authors:  Jianming Xie; Lei Wang; Ning Wu; Ansgar Brock; Glen Spraggon; Peter G Schultz
Journal:  Nat Biotechnol       Date:  2004-09-19       Impact factor: 54.908

9.  Control of protein phosphorylation with a genetically encoded photocaged amino acid.

Authors:  Edward A Lemke; Daniel Summerer; Bernhard H Geierstanger; Scott M Brittain; Peter G Schultz
Journal:  Nat Chem Biol       Date:  2007-10-28       Impact factor: 15.040

10.  A promiscuous aminoacyl-tRNA synthetase that incorporates cysteine, methionine, and alanine homologs into proteins.

Authors:  Eric Brustad; Mark L Bushey; Ansgar Brock; Johnathan Chittuluru; Peter G Schultz
Journal:  Bioorg Med Chem Lett       Date:  2008-09-17       Impact factor: 2.823
  10 in total
  16 in total

1.  A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum.

Authors:  Yane-Shih Wang; Xinqiang Fang; Ashley L Wallace; Bo Wu; Wenshe R Liu
Journal:  J Am Chem Soc       Date:  2012-02-06       Impact factor: 15.419

2.  An orthogonalized platform for genetic code expansion in both bacteria and eukaryotes.

Authors:  James S Italia; Partha Sarathi Addy; Chester J J Wrobel; Lisa A Crawford; Marc J Lajoie; Yunan Zheng; Abhishek Chatterjee
Journal:  Nat Chem Biol       Date:  2017-02-13       Impact factor: 15.040

3.  Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins.

Authors:  Rui Gan; Jessica G Perez; Erik D Carlson; Ioanna Ntai; Farren J Isaacs; Neil L Kelleher; Michael C Jewett
Journal:  Biotechnol Bioeng       Date:  2017-02-02       Impact factor: 4.530

4.  Importance of single molecular determinants in the fidelity of expanded genetic codes.

Authors:  Alicja K Antonczak; Zuzana Simova; Isaac T Yonemoto; Matthias Bochtler; Anna Piasecka; Honorata Czapinska; Andrea Brancale; Eric M Tippmann
Journal:  Proc Natl Acad Sci U S A       Date:  2011-01-11       Impact factor: 11.205

5.  Directed evolution as a powerful synthetic biology tool.

Authors:  Ryan E Cobb; Ning Sun; Huimin Zhao
Journal:  Methods       Date:  2012-03-23       Impact factor: 3.608

6.  Broadening the Toolkit for Quantitatively Evaluating Noncanonical Amino Acid Incorporation in Yeast.

Authors:  Jessica T Stieglitz; Kelly A Potts; James A Van Deventer
Journal:  ACS Synth Biol       Date:  2021-11-03       Impact factor: 5.110

Review 7.  Incorporation of Non-Canonical Amino Acids.

Authors:  Lilia Leisle; Francis Valiyaveetil; Ryan A Mehl; Christopher A Ahern
Journal:  Adv Exp Med Biol       Date:  2015       Impact factor: 2.622

8.  Unnatural amino acids: better than the real things?

Authors:  Ellen C Minnihan; Kenichi Yokoyama; Joanne Stubbe
Journal:  F1000 Biol Rep       Date:  2009-11-26

9.  Evolution of multiple, mutually orthogonal prolyl-tRNA synthetase/tRNA pairs for unnatural amino acid mutagenesis in Escherichia coli.

Authors:  Abhishek Chatterjee; Han Xiao; Peter G Schultz
Journal:  Proc Natl Acad Sci U S A       Date:  2012-08-27       Impact factor: 11.205

Review 10.  Playing with the Molecules of Life.

Authors:  Douglas D Young; Peter G Schultz
Journal:  ACS Chem Biol       Date:  2018-03-02       Impact factor: 5.100
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