| Literature DB >> 20513433 |
Mekonnen Lemma Dechassa1, Abdellah Sabri, Santhi Pondugula, Stefan R Kassabov, Nilanjana Chatterjee, Michael P Kladde, Blaine Bartholomew.
Abstract
The ATP-dependent chromatin remodeling complex SWI/SNF regulates transcription and has been implicated in promoter nucleosome eviction. Efficient nucleosome disassembly by SWI/SNF alone in biochemical assays, however, has not been directly observed. Employing a model system of dinucleosomes rather than mononucleosomes, we demonstrate that remodeling leads to ordered and efficient disassembly of one of the two nucleosomes. An H2A/H2B dimer is first rapidly displaced, and then, in a slower reaction, an entire histone octamer is lost. Nucleosome disassembly by SWI/SNF did not require additional factors such as chaperones or acceptors of histones. Observations in single molecules as well as bulk measurement suggest that a key intermediate in this process is one in which a nucleosome is moved toward the adjacent nucleosome. SWI/SNF recruited by the transcriptional activator Gal4-VP16 preferentially mobilizes the proximal nucleosome and destabilizes the adjacent nucleosome. Copyright 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20513433 PMCID： PMC3161732 DOI： 10.1016/j.molcel.2010.02.040
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970