Literature DB >> 20418878

A direct NMR method for the measurement of competitive kinetic isotope effects.

Jefferson Chan1, Andrew R Lewis, Michel Gilbert, Marie-France Karwaski, Andrew J Bennet.   

Abstract

We present a technique that uses (13)C NMR spectroscopy to measure kinetic isotope effects on the second-order rate constant (k(cat)/K(m)) for enzyme-catalyzed reactions. Using only milligram quantities of isotopically labeled substrates, precise competitive KIEs can be determined while following the ongoing reaction directly in a NMR spectrometer. Our results for the Vibrio cholerae sialidase-catalyzed hydrolysis of natural substrate analogs support a concerted enzymatic transition state for these reactions.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20418878     DOI： 10.1038/nchembio.352

Source DB:  PubMed          Journal:  Nat Chem Biol        ISSN： 1552-4450            Impact factor:   15.040


  14 in total

1.  Glycosidase mechanisms: anatomy of a finely tuned catalyst.

Authors:  D L Zechel; S G Withers
Journal:  Acc Chem Res       Date:  2000-01       Impact factor: 22.384

2.  Mutagenesis of the conserved active-site tyrosine changes a retaining sialidase into an inverting sialidase.

Authors:  Jacqueline N Watson; Veedeeta Dookhun; Thor J Borgford; Andrew J Bennet
Journal:  Biochemistry       Date:  2003-11-04       Impact factor: 3.162

Review 3.  Mechanistic insights into glycosidase chemistry.

Authors:  David J Vocadlo; Gideon J Davies
Journal:  Curr Opin Chem Biol       Date:  2008-10       Impact factor: 8.822

4.  Structural and kinetic analysis of two covalent sialosyl-enzyme intermediates on Trypanosoma rangeli sialidase.

Authors:  Andrew G Watts; Pablo Oppezzo; Stephen G Withers; Pedro M Alzari; Alejandro Buschiazzo
Journal:  J Biol Chem       Date:  2005-11-18       Impact factor: 5.157

5.  A kinetic isotope effect study on the hydrolysis reactions of methyl xylopyranosides and methyl 5-thioxylopyranosides: oxygen versus sulfur stabilization of carbenium ions.

Authors:  D Indurugalla; A J Bennet
Journal:  J Am Chem Soc       Date:  2001-11-07       Impact factor: 15.419

6.  Equilibrium perturbation by isotope substitution.

Authors:  M I Schimerlik; J E Rife; W W Cleland
Journal:  Biochemistry       Date:  1975-12-02       Impact factor: 3.162

7.  Transition State Structure for the Hydrolysis of NAD Catalyzed by Diphtheria Toxin.

Authors:  Paul J Berti; Steven R Blanke; Vern L Schramm
Journal:  J Am Chem Soc       Date:  1997-12-17       Impact factor: 15.419

8.  Acyclic ribooxacarbenium ion mimics as transition state analogues of human and malarial purine nucleoside phosphorylases.

Authors:  Erika A Taylor; Keith Clinch; Peter M Kelly; Lei Li; Gary B Evans; Peter C Tyler; Vern L Schramm
Journal:  J Am Chem Soc       Date:  2007-05-11       Impact factor: 15.419

9.  Transition state structures for the hydrolysis of alpha-D-glucopyranosyl fluoride by retaining and inverting reactions of glycosylases.

Authors:  Y Tanaka; W Tao; J S Blanchard; E J Hehre
Journal:  J Biol Chem       Date:  1994-12-23       Impact factor: 5.157

10.  The specificity of viral and bacterial sialidases for alpha(2-3)- and alpha(2-6)-linked sialic acids in glycoproteins.

Authors:  A P Corfield; H Higa; J C Paulson; R Schauer
Journal:  Biochim Biophys Acta       Date:  1983-04-28
View more
  21 in total

1.  Synthesis of Site-Specifically 13C Labeled Linoleic Acids.

Authors:  Adam R Offenbacher; Hui Zhu; Judith P Klinman
Journal:  Tetrahedron Lett       Date:  2016-08-22       Impact factor: 2.415

2.  Determination of hepatitis delta virus ribozyme N(-1) nucleobase and functional group specificity using internal competition kinetics.

Authors:  Daniel L Kellerman; Kandice S Simmons; Mayra Pedraza; Joseph A Piccirilli; Darrin M York; Michael E Harris
Journal:  Anal Biochem       Date:  2015-05-01       Impact factor: 3.365

Review 3.  Enzymatic transition states, transition-state analogs, dynamics, thermodynamics, and lifetimes.

Authors:  Vern L Schramm
Journal:  Annu Rev Biochem       Date:  2011       Impact factor: 23.643

4.  Mechanistic evidence for a front-side, SNi-type reaction in a retaining glycosyltransferase.

Authors:  Seung Seo Lee; Sung You Hong; James C Errey; Atsushi Izumi; Gideon J Davies; Benjamin G Davis
Journal:  Nat Chem Biol       Date:  2011-08-07       Impact factor: 15.040

5.  13C kinetic isotope effects on the reaction of a flavin amine oxidase determined from whole molecule isotope effects.

Authors:  José R Tormos; Marina B Suarez; Paul F Fitzpatrick
Journal:  Arch Biochem Biophys       Date:  2016-11-01       Impact factor: 4.013

6.  Sensitive and Accurate 13C Kinetic Isotope Effect Measurements Enabled by Polarization Transfer.

Authors:  Eugene E Kwan; Yongho Park; Harrison A Besser; Thayer L Anderson; Eric N Jacobsen
Journal:  J Am Chem Soc       Date:  2016-12-29       Impact factor: 15.419

7.  18O Kinetic Isotope Effects Reveal an Associative Transition State for Phosphite Dehydrogenase Catalyzed Phosphoryl Transfer.

Authors:  Graeme W Howe; Wilfred A van der Donk
Journal:  J Am Chem Soc       Date:  2018-12-12       Impact factor: 15.419

8.  Kinetic isotope effects reveal early transition state of protein lysine methyltransferase SET8.

Authors:  Joshua A Linscott; Kanishk Kapilashrami; Zhen Wang; Chamara Senevirathne; Ian R Bothwell; Gil Blum; Minkui Luo
Journal:  Proc Natl Acad Sci U S A       Date:  2016-12-09       Impact factor: 11.205

Review 9.  Measuring specificity in multi-substrate/product systems as a tool to investigate selectivity in vivo.

Authors:  Yin-Ming Kuo; Ryan A Henry; Andrew J Andrews
Journal:  Biochim Biophys Acta       Date:  2015-08-29

10.  Determination of relative rate constants for in vitro RNA processing reactions by internal competition.

Authors:  Hsuan-Chun Lin; Lindsay E Yandek; Ino Gjermeni; Michael E Harris
Journal:  Anal Biochem       Date:  2014-08-28       Impact factor: 3.365
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.