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Literature DB >> 18806790

Tracking the structural dynamics of proteins in solution using time-resolved wide-angle X-ray scattering.

Marco Cammarata¹, Matteo Levantino, Friedrich Schotte, Philip A Anfinrud, Friederike Ewald, Jungkweon Choi, Antonio Cupane, Michael Wulff, Hyotcherl Ihee.

Abstract

We demonstrate tracking of protein structural changes with time-resolved wide-angle X-ray scattering (TR-WAXS) with nanosecond time resolution. We investigated the tertiary and quaternary conformational changes of human hemoglobin under nearly physiological conditions triggered by laser-induced ligand photolysis. We also report data on optically induced tertiary relaxations of myoglobin and refolding of cytochrome c to illustrate the wide applicability of the technique. By providing insights into the structural dynamics of proteins functioning in their natural environment, TR-WAXS complements and extends results obtained with time-resolved optical spectroscopy and X-ray crystallography.

Entities: Chemical Disease Gene Species

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Year: 2008 PMID： 18806790 PMCID： PMC3159148 DOI： 10.1038/nmeth.1255

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

26 in total

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Authors: Shuji Akiyama; Satoshi Takahashi; Tetsunari Kimura; Koichiro Ishimori; Isao Morishima; Yukihiro Nishikawa; Tetsuro Fujisawa
Journal: Proc Natl Acad Sci U S A Date: 2002-01-02 Impact factor: 11.205

9. Time-resolved absorption and UV resonance Raman spectra reveal stepwise formation of T quaternary contacts in the allosteric pathway of hemoglobin.

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Journal: J Mol Biol Date: 2004-07-16 Impact factor: 5.469

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Authors: Friedrich Schotte; Manho Lim; Timothy A Jackson; Aleksandr V Smirnov; Jayashree Soman; John S Olson; George N Phillips; Michael Wulff; Philip A Anfinrud
Journal: Science Date: 2003-06-20 Impact factor: 47.728

85 in total

1. Cooperative macromolecular device revealed by meta-analysis of static and time-resolved structures.

Authors: Zhong Ren; Vukica Srajer; James E Knapp; William E Royer
Journal: Proc Natl Acad Sci U S A Date: 2011-12-14 Impact factor: 11.205

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Authors: Haydyn D T Mertens; Alen Piljić; Carsten Schultz; Dmitri I Svergun
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Journal: Proc Natl Acad Sci U S A Date: 2010-04-06 Impact factor: 11.205

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Authors: Sebastian Westenhoff; Erik Malmerberg; David Arnlund; Linda Johansson; Elena Nazarenko; Marco Cammarata; Jan Davidsson; Vincent Chaptal; Jeff Abramson; Gergely Katona; Andreas Menzel; Richard Neutze
Journal: Nat Methods Date: 2010-09-29 Impact factor: 28.547

5. Insulin hexamer dissociation dynamics revealed by photoinduced T-jumps and time-resolved X-ray solution scattering.

Authors: Dolev Rimmerman; Denis Leshchev; Darren J Hsu; Jiyun Hong; Baxter Abraham; Irina Kosheleva; Robert Henning; Lin X Chen
Journal: Photochem Photobiol Sci Date: 2018-07-11 Impact factor: 3.982

Review 6. X-ray scattering combined with coordinate-based analyses for applications in natural and artificial photosynthesis.

Authors: David M Tiede; Kristy L Mardis; Xiaobing Zuo
Journal: Photosynth Res Date: 2009 Nov-Dec Impact factor: 3.573

10. Dynamics of Quaternary Structure Transitions in R-State Carbonmonoxyhemoglobin Unveiled in Time-Resolved X-ray Scattering Patterns Following a Temperature Jump.

Authors: Hyun Sun Cho; Friedrich Schotte; Valentyn Stadnytskyi; Anthony DiChiara; Robert Henning; Philip Anfinrud
Journal: J Phys Chem B Date: 2018-10-16 Impact factor: 2.991