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Literature DB >> 12091726

Structural hierarchy of several proteins observed by wide-angle solution scattering.

Mitsuhiro Hirai¹, Hiroki Iwase, Tomohiro Hayakawa, Keiko Miura, Katsuaki Inoue.

Abstract

In the present study using a high-intensity X-ray beam from a third-generation synchrotron radiation source, it is demonstrated that a wide-angle X-ray scattering (WAXS) profile from several globular proteins in solution can reflect not only the overall structures (approximately 300 A distance resolution) but also intramolecular structures ranging to secondary structures (approximately 2.5 A distance resolution). The proteins treated in the present experiments are classified as different types of structure categories, namely, as all-alpha, all-beta and alpha + beta proteins. Here the full-range experimental scattering curves are compared with the theoretical curves, suggesting a further availability of the SR-WAXS method for studies of structure hierarchy and the function of proteins in solutions.

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Year: 2002 PMID： 12091726 DOI： 10.1107/s0909049502006593

Source DB: PubMed Journal: J Synchrotron Radiat ISSN： 0909-0495 Impact factor: 2.616

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Cited

15 in total

1. Kinetic asymmetry of subunit exchange of homooligomeric protein as revealed by deuteration-assisted small-angle neutron scattering.

Authors: Masaaki Sugiyama; Eiji Kurimoto; Hirokazu Yagi; Kazuhiro Mori; Toshiharu Fukunaga; Mitsuhiro Hirai; Giuseppe Zaccai; Koichi Kato
Journal: Biophys J Date: 2011-10-19 Impact factor: 4.033

2. X-ray diffraction "fingerprinting" of DNA structure in solution for quantitative evaluation of molecular dynamics simulation.

Authors: Xiaobing Zuo; Guanglei Cui; Kenneth M Merz; Ligang Zhang; Frederick D Lewis; David M Tiede
Journal: Proc Natl Acad Sci U S A Date: 2006-02-27 Impact factor: 11.205

3. Molecular crowding inhibits intramolecular breathing motions in proteins.

Authors: Lee Makowski; Diane J Rodi; Suneeta Mandava; David D L Minh; David B Gore; Robert F Fischetti
Journal: J Mol Biol Date: 2007-08-17 Impact factor: 5.469

4. A rapid coarse residue-based computational method for x-ray solution scattering characterization of protein folds and multiple conformational states of large protein complexes.

Authors: Sichun Yang; Sanghyun Park; Lee Makowski; Benoît Roux
Journal: Biophys J Date: 2009-06-03 Impact factor: 4.033

10. Reverse conformational changes of the light chain-binding domain of myosin V and VI processive motor heads during and after hydrolysis of ATP by small-angle X-ray solution scattering.

Authors: Yasunobu Sugimoto; Osamu Sato; Shinya Watanabe; Reiko Ikebe; Mitsuo Ikebe; Katsuzo Wakabayashi
Journal: J Mol Biol Date: 2009-07-14 Impact factor: 5.469

Structural hierarchy of several proteins observed by wide-angle solution scattering.

1. Kinetic asymmetry of subunit exchange of homooligomeric protein as revealed by deuteration-assisted small-angle neutron scattering.

2. X-ray diffraction "fingerprinting" of DNA structure in solution for quantitative evaluation of molecular dynamics simulation.

3. Molecular crowding inhibits intramolecular breathing motions in proteins.

4. A rapid coarse residue-based computational method for x-ray solution scattering characterization of protein folds and multiple conformational states of large protein complexes.

5. Change of dynamics of raft-model membrane induced by amyloid-β protein binding.

6. Coherent neutron scattering and collective dynamics in the protein, GFP.

7. Tracking the structural dynamics of proteins in solution using time-resolved wide-angle X-ray scattering.

8. SoftWAXS: a computational tool for modeling wide-angle X-ray solution scattering from biomolecules.

9. Direct Evidence for the Effect of Glycerol on Protein Hydration and Thermal Structural Transition.

10. Reverse conformational changes of the light chain-binding domain of myosin V and VI processive motor heads during and after hydrolysis of ATP by small-angle X-ray solution scattering.