Literature DB >> 1841710

Measurement of 15N-13C J couplings in staphylococcal nuclease.

F Delaglio1, D A Torchia, A Bax.   

Abstract

15N-C alpha and 15N-C' J couplings were measured for the backbone of staphylococcal nuclease, uniformly enriched with 15N and 13C. It is found that the 1JC'N coupling is similar for beta-sheet, J = 14.8 +/- 0.5 and for alpha-helix, J = 14.8 +/- 0.4 but tends to be larger for the unstructured N- and C-terminal ends of the protein (J = 15.6 +/- 0.5). On average, 1JNC alpha are smaller for alpha-helical residues (J = 9.6 +/- 0.3 Hz) compared to beta-sheet (J = 10.9 +/- 0.8 Hz) and a substantial difference is observed for 2JNC alpha in alpha-helices (J = 6.4 +/- 0.4 Hz) and beta-sheets (J = 8.3 +/- 0.8 Hz).

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Year:  1991        PMID： 1841710     DOI： 10.1007/bf02192865

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  8 in total

1.  Two-dimensional NMR studies of staphylococcal nuclease. 1. Sequence-specific assignments of hydrogen-1 signals and solution structure of the nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+ ternary complex.

Authors:  J F Wang; D M LeMaster; J L Markley
Journal:  Biochemistry       Date:  1990-01-09       Impact factor: 3.162

2.  The crystal structure of the ternary complex of staphylococcal nuclease, Ca2+, and the inhibitor pdTp, refined at 1.65 A.

Authors:  P J Loll; E E Lattman
Journal:  Proteins       Date:  1989

3.  Staphylococcal nuclease: sequential assignments and solution structure.

Authors:  D A Torchia; S W Sparks; A Bax
Journal:  Biochemistry       Date:  1989-06-27       Impact factor: 3.162

4.  Deletion of the omega-loop in the active site of staphylococcal nuclease. 2. Effects on protein structure and dynamics.

Authors:  D M Baldisseri; D A Torchia; L B Poole; J A Gerlt
Journal:  Biochemistry       Date:  1991-04-16       Impact factor: 3.162

5.  Local structural features around the C-terminal segment of Streptomyces subtilisin inhibitor studied by carbonyl carbon nuclear magnetic resonances three phenylalanyl residues.

Authors:  M Kainosho; H Nagao; T Tsuji
Journal:  Biochemistry       Date:  1987-02-24       Impact factor: 3.162

6.  Two-dimensional NMR studies of staphylococcal nuclease. 2. Sequence-specific assignments of carbon-13 and nitrogen-15 signals from the nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+ ternary complex.

Authors:  J F Wang; A P Hinck; S N Loh; J L Markley
Journal:  Biochemistry       Date:  1990-01-09       Impact factor: 3.162

7.  Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease.

Authors:  L E Kay; D A Torchia; A Bax
Journal:  Biochemistry       Date:  1989-11-14       Impact factor: 3.162

8.  A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.

Authors:  M Ikura; L E Kay; A Bax
Journal:  Biochemistry       Date:  1990-05-15       Impact factor: 3.162
  8 in total
  42 in total

1.  Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings.

Authors:  P Permi; A Annila
Journal:  J Biomol NMR       Date:  2000-03       Impact factor: 2.835

2.  H-bonding mediates polarization of peptide groups in folded proteins.

Authors:  Nenad Juranić; Slobodan Macura; Franklyn G Prendergast
Journal:  Protein Sci       Date:  2003-11       Impact factor: 6.725

3.  Angular dependence of 1J(Ni,Calphai) and 2J(Ni,Calpha(i-1)) coupling constants measured in J-modulated HSQCs.

Authors:  Julia Wirmer; Harald Schwalbe
Journal:  J Biomol NMR       Date:  2002-05       Impact factor: 2.835

4.  Simultaneous measurement of protein one-bond and two-bond nitrogen-carbon coupling constants using an internally referenced quantitative J-correlated [(15)N,(1)H]-TROSY-HNC experiment.

Authors:  Hans L J Wienk; Mitcheell M Martínez; Gary N Yalloway; Jürgen M Schmidt; Carlos Pérez; Heinz Rüterjans; Frank Löhr
Journal:  J Biomol NMR       Date:  2003-02       Impact factor: 2.835

5.  Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction.

Authors:  Alessandra Romanelli; Alexander Shekhtman; David Cowburn; Tom W Muir
Journal:  Proc Natl Acad Sci U S A       Date:  2004-04-15       Impact factor: 11.205

6.  Measurement of two-bond JCOH alpha coupling constants in proteins uniformly enriched with 13C.

Authors:  G W Vuister; A Bax
Journal:  J Biomol NMR       Date:  1992-07       Impact factor: 2.835

7.  A 4D TROSY-based pulse scheme for correlating 1HNi,15Ni,13Calphai,13C'i-1 chemical shifts in high molecular weight, 15N,13C, 2H labeled proteins.

Authors:  R Konrat; D Yang; L E Kay
Journal:  J Biomol NMR       Date:  1999-12       Impact factor: 2.835

8.  HA-detected experiments for the backbone assignment of intrinsically disordered proteins.

Authors:  Sampo Mäntylahti; Olli Aitio; Maarit Hellman; Perttu Permi
Journal:  J Biomol NMR       Date:  2010-05-01       Impact factor: 2.835

9.  Intraresidual HNCA: an experiment for correlating only intraresidual backbone resonances.

Authors:  Perttu Permi
Journal:  J Biomol NMR       Date:  2002-07       Impact factor: 2.835

10.  Sequential backbone assignment of isotopically enriched proteins in D2O by deuterium-decoupled HA(CA)N and HA(CACO)N.

Authors:  A C Wang; S Grzesiek; R Tschudin; P J Lodi; A Bax
Journal:  J Biomol NMR       Date:  1995-06       Impact factor: 2.835
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