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Literature DB >> 17977018

A dose-rate effect in single-particle electron microscopy.

James Z Chen¹, Carsten Sachse, Chen Xu, Thorsten Mielke, Christian M T Spahn, Nikolaus Grigorieff.

Abstract

A low beam intensity, low electron dose imaging method has been developed for single-particle electron cryo-microscopy (cryo-EM). Experiments indicate that the new technique can reduce beam-induced specimen movement and secondary radiolytic effects, such as "bubbling". The improvement in image quality, especially for multiple-exposure data collection, will help single-particle cryo-EM to reach higher resolution.

Entities: Chemical

Mesh：

Year: 2007 PMID： 17977018 PMCID： PMC2213720 DOI： 10.1016/j.jsb.2007.09.017

Source DB: PubMed Journal: J Struct Biol ISSN： 1047-8477 Impact factor: 2.867

23 in total

Review 1. Single-particle imaging of macromolecules by cryo-electron microscopy.

Authors: Joachim Frank
Journal: Annu Rev Biophys Biomol Struct Date: 2001-10-25

2. Stroboscopic image capture: reducing the dose per frame by a factor of 30 does not prevent beam-induced specimen movement in paraffin.

Authors: Dieter Typke; Christopher J Gilpin; Kenneth H Downing; Robert M Glaeser
Journal: Ultramicroscopy Date: 2006-07-24 Impact factor: 2.689

3. A comparison of liquid nitrogen and liquid helium as cryogens for electron cryotomography.

Authors: Cristina V Iancu; Elizabeth R Wright; J Bernard Heymann; Grant J Jensen
Journal: J Struct Biol Date: 2006-01-04 Impact factor: 2.867

4. The orthogonal tilt reconstruction method: an approach to generating single-class volumes with no missing cone for ab initio reconstruction of asymmetric particles.

Authors: Andres E Leschziner; Eva Nogales
Journal: J Struct Biol Date: 2005-12-21 Impact factor: 2.867

Review 5. Cryo-electron microscopy of vitrified specimens.

Authors: J Dubochet; M Adrian; J J Chang; J C Homo; J Lepault; A W McDowall; P Schultz
Journal: Q Rev Biophys Date: 1988-05 Impact factor: 5.318

Review 6. Radiation damage in macromolecular cryocrystallography.

Authors: Raimond B G Ravelli; Elspeth F Garman
Journal: Curr Opin Struct Biol Date: 2006-08-30 Impact factor: 6.809

7. Three-dimensional structure of bovine NADH:ubiquinone oxidoreductase (complex I) at 22 A in ice.

Authors: N Grigorieff
Journal: J Mol Biol Date: 1998-04-17 Impact factor: 5.469

8. Assessment of electron irradiation damage to biomolecules by electron diffraction and electron energy-loss spectroscopy.

Authors: M Misra; R F Egerton
Journal: Ultramicroscopy Date: 1984 Impact factor: 2.689

9. Three-dimensional reconstruction from a single-exposure, random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli.

Authors: M Radermacher; T Wagenknecht; A Verschoor; J Frank
Journal: J Microsc Date: 1987-05 Impact factor: 1.758

10. Visualization of protein-nucleic acid interactions in a virus. Refined structure of intact tobacco mosaic virus at 2.9 A resolution by X-ray fiber diffraction.

Authors: K Namba; R Pattanayek; G Stubbs
Journal: J Mol Biol Date: 1989-07-20 Impact factor: 5.469

18 in total

A dose-rate effect in single-particle electron microscopy.

Review 1. Single-particle imaging of macromolecules by cryo-electron microscopy.

2. Stroboscopic image capture: reducing the dose per frame by a factor of 30 does not prevent beam-induced specimen movement in paraffin.

3. A comparison of liquid nitrogen and liquid helium as cryogens for electron cryotomography.

4. The orthogonal tilt reconstruction method: an approach to generating single-class volumes with no missing cone for ab initio reconstruction of asymmetric particles.

Review 5. Cryo-electron microscopy of vitrified specimens.

Review 6. Radiation damage in macromolecular cryocrystallography.

7. Three-dimensional structure of bovine NADH:ubiquinone oxidoreductase (complex I) at 22 A in ice.

8. Assessment of electron irradiation damage to biomolecules by electron diffraction and electron energy-loss spectroscopy.

9. Three-dimensional reconstruction from a single-exposure, random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli.

10. Visualization of protein-nucleic acid interactions in a virus. Refined structure of intact tobacco mosaic virus at 2.9 A resolution by X-ray fiber diffraction.

1. A test-bed for optimizing high-resolution single particle reconstructions.

Review 2. Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy.

Review 3. Electron Tomography: A Three-Dimensional Analytic Tool for Hard and Soft Materials Research.

4. Cryomesh: a new substrate for cryo-electron microscopy.

5. Protein secondary structure determination by constrained single-particle cryo-electron tomography.

6. A fast cross-validation method for alignment of electron tomography images based on Beer-Lambert law.

7. Radiation damage in single-particle cryo-electron microscopy: effects of dose and dose rate.

Review 8. Molecular electron microscopy: state of the art and current challenges.

9. Beam-induced motion of vitrified specimen on holey carbon film.

10. Non-rigid image registration to reduce beam-induced blurring of cryo-electron microscopy images.