Literature DB >> 17567791

Detection of Clostridium difficile toxin: comparison of enzyme immunoassay results with results obtained by cytotoxicity assay.

Daniel M Musher1, Atisha Manhas, Pranav Jain, Franziska Nuila, Amna Waqar, Nancy Logan, Bernard Marino, Edward A Graviss.   

Abstract

Several kinds of laboratory techniques are available to detect Clostridium difficile toxin in fecal samples. Because questions have been raised about the reliability of immunoassays compared to the accepted standard, cytotoxicity assay, we studied three enzyme immunoassays (EIAs) and one rapid EIA, which demonstrated relatively good sensitivities and specificities compared to cytotoxicity assay.

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Year:  2007        PMID： 17567791      PMCID： PMC1951241          DOI： 10.1128/JCM.00686-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

Review 1.  Clinical practice. Antibiotic-associated diarrhea.

Authors:  John G Bartlett
Journal:  N Engl J Med       Date:  2002-01-31       Impact factor: 91.245

2.  Clinical usefulness of components of the Triage immunoassay, enzyme immunoassay for toxins A and B, and cytotoxin B tissue culture assay for the diagnosis of Clostridium difficile diarrhea.

Authors:  Viki Massey; Daniel B Gregson; Abdul H Chagla; Miriam Storey; Michael A John; Zafar Hussain
Journal:  Am J Clin Pathol       Date:  2003-01       Impact factor: 2.493

3.  An epidemic, toxin gene-variant strain of Clostridium difficile.

Authors:  L Clifford McDonald; George E Killgore; Angela Thompson; Robert C Owens; Sophia V Kazakova; Susan P Sambol; Stuart Johnson; Dale N Gerding
Journal:  N Engl J Med       Date:  2005-12-01       Impact factor: 91.245

4.  Evaluation of methods for detection of toxins in specimens of feces submitted for diagnosis of Clostridium difficile-associated diarrhea.

Authors:  D O'Connor; P Hynes; M Cormican; E Collins; G Corbett-Feeney; M Cassidy
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

5.  Comparison of the TOX A/B test to a cell culture cytotoxicity assay for the detection of Clostridium difficile in stools.

Authors:  W E Aldeen; M Bingham; A Aiderzada; J Kucera; S Jense; K C Carroll
Journal:  Diagn Microbiol Infect Dis       Date:  2000-04       Impact factor: 2.803

6.  Evaluation of three commercial enzyme immunoassay kits for detecting faecal Clostridium difficile toxins.

Authors:  S A Arrow; L Croese; R A Bowman; T V Riley
Journal:  J Clin Pathol       Date:  1994-10       Impact factor: 3.411

7.  Six rapid tests for direct detection of Clostridium difficile and its toxins in fecal samples compared with the fibroblast cytotoxicity assay.

Authors:  David K Turgeon; Thomas J Novicki; John Quick; LaDonna Carlson; Pat Miller; Bruce Ulness; Anne Cent; Rhoda Ashley; Ann Larson; Marie Coyle; Ajit P Limaye; Brad T Cookson; Thomas R Fritsche
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

8.  Comparison of four commercially available rapid enzyme immunoassays with cytotoxin assay for detection of Clostridium difficile toxin(s) from stool specimens.

Authors:  C S Merz; C Kramer; M Forman; L Gluck; K Mills; K Senft; I Steiman; N Wallace; P Charache
Journal:  J Clin Microbiol       Date:  1994-05       Impact factor: 5.948

9.  The diagnosis of Clostridium difficile-associated diarrhea: comparison of Triage C. difficile panel, EIA for Tox A/B and cytotoxin assays.

Authors:  M J Alfa; B Swan; B VanDekerkhove; P Pang; G K M Harding
Journal:  Diagn Microbiol Infect Dis       Date:  2002-08       Impact factor: 2.803

10.  Detection of toxin production in Clostridium difficile strains by three different methods.

Authors:  M Yücesoy; J McCoubrey; R Brown; I R Poxton
Journal:  Clin Microbiol Infect       Date:  2002-07       Impact factor: 8.067
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  20 in total

1.  Comparison of two commercial molecular assays to a laboratory-developed molecular assay for diagnosis of Clostridium difficile infection.

Authors:  Tess Karre; Lynne Sloan; Robin Patel; Jayawant Mandrekar; Jon Rosenblatt
Journal:  J Clin Microbiol       Date:  2010-12-01       Impact factor: 5.948

2.  Rapid and sensitive loop-mediated isothermal amplification test for Clostridium difficile detection challenges cytotoxin B cell test and culture as gold standard.

Authors:  Torbjörn Norén; Ingegärd Alriksson; Josefin Andersson; Thomas Akerlund; Magnus Unemo
Journal:  J Clin Microbiol       Date:  2010-11-24       Impact factor: 5.948

3.  Clostridioides difficile laboratory diagnostic techniques: a comparative approach of rapid and molecular methods.

Authors:  Thais Simões Camargo; Moacyr Silva Junior; Luis Fernando Aranha Camargo; Veronica Pivetta Biotto; André Mario Doi; Paula Celia Mariko Koga; Carolina Nunes França; Marines Dalla Valle Martino
Journal:  Arch Microbiol       Date:  2021-01-18       Impact factor: 2.552

4.  C. Diff Quik Chek complete enzyme immunoassay provides a reliable first-line method for detection of Clostridium difficile in stool specimens.

Authors:  Criziel D Quinn; Susan E Sefers; Wisal Babiker; Ying He; Romina Alcabasa; Charles W Stratton; Karen C Carroll; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2009-12-02       Impact factor: 5.948

Review 5.  Laboratory diagnosis of Clostridium difficile infection can molecular amplification methods move us out of uncertainty?

Authors:  Fred C Tenover; Ellen Jo Baron; Lance R Peterson; David H Persing
Journal:  J Mol Diagn       Date:  2011-08-18       Impact factor: 5.568

Review 6.  Laboratory Tests for the Diagnosis of Clostridium difficile.

Authors:  Karen C Carroll; Masako Mizusawa
Journal:  Clin Colon Rectal Surg       Date:  2020-02-25

7.  Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples.

Authors:  Paul D Stamper; Romina Alcabasa; Deborah Aird; Wisal Babiker; Jennifer Wehrlin; Ijeoma Ikpeama; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2008-12-10       Impact factor: 5.948

8.  Univariate and multivariate analysis of risk factors for severe Clostridium difficile-associated diarrhoea: importance of co-morbidity and serum C-reactive protein.

Authors:  Christian Hardt; Thomas Berns; Wolfgang Treder; Franz-Ludwig Dumoulin
Journal:  World J Gastroenterol       Date:  2008-07-21       Impact factor: 5.742

9.  Algorithm combining toxin immunoassay and stool culture for diagnosis of Clostridium difficile infection.

Authors:  Bo-Moon Shin; Eun Young Kuak; Eun Joo Lee; J Glenn Songer
Journal:  J Clin Microbiol       Date:  2009-07-22       Impact factor: 5.948

10.  Comparison of three commercial methods for rapid detection of Clostridium difficile toxins A and B from fecal specimens.

Authors:  L Alcalá; L Sánchez-Cambronero; M P Catalán; M Sánchez-Somolinos; M T Peláez; M Marín; E Bouza
Journal:  J Clin Microbiol       Date:  2008-09-10       Impact factor: 5.948
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