Literature DB >> 21106782

Rapid and sensitive loop-mediated isothermal amplification test for Clostridium difficile detection challenges cytotoxin B cell test and culture as gold standard.

Torbjörn Norén1, Ingegärd Alriksson, Josefin Andersson, Thomas Akerlund, Magnus Unemo.   

Abstract

Compared to the composite gold standard cytotoxin B assay and toxigenic culture, the loop-mediated isothermal amplification (LAMP) test for Clostridium difficile had a sensitivity and specificity of 98%, positive predictive value of 92%, and negative predictive value of >99%. A one-hour turnaround time for the LAMP test provides rapid diagnosis and cost savings.

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Year:  2010        PMID： 21106782      PMCID： PMC3043499          DOI： 10.1128/JCM.01824-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

1.  Comparison of two rapid assays for Clostridium difficile Common antigen and a C difficile toxin A/B assay with the cell culture neutralization assay.

Authors:  Megan E Reller; Romina C Alcabasa; Clara A Lema; Karen C Carroll
Journal:  Am J Clin Pathol       Date:  2010-01       Impact factor: 2.493

2.  Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples.

Authors:  Paul D Stamper; Romina Alcabasa; Deborah Aird; Wisal Babiker; Jennifer Wehrlin; Ijeoma Ikpeama; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2008-12-10       Impact factor: 5.948

3.  Induction of toxins in Clostridium difficile is associated with dramatic changes of its metabolism.

Authors:  Sture Karlsson; Lars G Burman; Thomas Åkerlund
Journal:  Microbiology       Date:  2008-11       Impact factor: 2.777

Review 4.  European Society of Clinical Microbiology and Infectious Diseases (ESCMID): data review and recommendations for diagnosing Clostridium difficile-infection (CDI).

Authors:  M J T Crobach; O M Dekkers; M H Wilcox; E J Kuijper
Journal:  Clin Microbiol Infect       Date:  2009-12       Impact factor: 8.067

5.  Comparison of BD GeneOhm Cdiff real-time PCR assay with a two-step algorithm and a toxin A/B enzyme-linked immunosorbent assay for diagnosis of toxigenic Clostridium difficile infection.

Authors:  Elizabeth J Kvach; David Ferguson; Paul F Riska; Marie L Landry
Journal:  J Clin Microbiol       Date:  2009-10-28       Impact factor: 5.948

Review 6.  Clinical recognition and diagnosis of Clostridium difficile infection.

Authors:  John G Bartlett; Dale N Gerding
Journal:  Clin Infect Dis       Date:  2008-01-15       Impact factor: 9.079

Review 7.  Clostridium difficile testing algorithms: what is practical and feasible?

Authors:  Monica L Schmidt; Peter H Gilligan
Journal:  Anaerobe       Date:  2009-10-22       Impact factor: 3.331

8.  Comparison of a rapid molecular method, the BD GeneOhm Cdiff assay, to the most frequently used laboratory tests for detection of toxin-producing Clostridium difficile in diarrheal feces.

Authors:  Gabriella Terhes; Edit Urbán; József Sóki; Eniko Nacsa; Elisabeth Nagy
Journal:  J Clin Microbiol       Date:  2009-09-30       Impact factor: 5.948

9.  Is a two-step glutamate dehyrogenase antigen-cytotoxicity neutralization assay algorithm superior to the premier toxin A and B enzyme immunoassay for laboratory detection of Clostridium difficile?

Authors:  Peter H Gilligan
Journal:  J Clin Microbiol       Date:  2008-02-06       Impact factor: 5.948

10.  Toxin B is essential for virulence of Clostridium difficile.

Authors:  Dena Lyras; Jennifer R O'Connor; Pauline M Howarth; Susan P Sambol; Glen P Carter; Tongted Phumoonna; Rachael Poon; Vicki Adams; Gayatri Vedantam; Stuart Johnson; Dale N Gerding; Julian I Rood
Journal:  Nature       Date:  2009-03-01       Impact factor: 49.962
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  27 in total

Review 1.  Molecular techniques for diagnosis of Clostridium difficile infection: systematic review and meta-analysis.

Authors:  John C O'Horo; Amy Jones; Matthew Sternke; Christopher Harper; Nasia Safdar
Journal:  Mayo Clin Proc       Date:  2012-07       Impact factor: 7.616

2.  Detection of toxigenic Clostridium difficile: comparison of the cell culture neutralization, Xpert C. difficile, Xpert C. difficile/Epi, and Illumigene C. difficile assays.

Authors:  P Pancholi; C Kelly; M Raczkowski; J M Balada-Llasat
Journal:  J Clin Microbiol       Date:  2012-01-25       Impact factor: 5.948

3.  Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices.

Authors:  M R Williams; R D Stedtfeld; H Waseem; T Stedtfeld; B Upham; W Khalife; B Etchebarne; M Hughes; J M Tiedje; S A Hashsham
Journal:  Anal Methods       Date:  2017-01-31       Impact factor: 2.896

Review 4.  Laboratory diagnosis of Clostridium difficile infection can molecular amplification methods move us out of uncertainty?

Authors:  Fred C Tenover; Ellen Jo Baron; Lance R Peterson; David H Persing
Journal:  J Mol Diagn       Date:  2011-08-18       Impact factor: 5.568

5.  Targeting the tcdA gene: is this appropriate for detection of A and/or B Clostridium difficile toxin-producing strains?

Authors:  Ken Kozak; Vecheslav Elagin; Torbjörn Norén; Magnus Unemo
Journal:  J Clin Microbiol       Date:  2011-06       Impact factor: 5.948

6.  Reply to "Comparison of detection methods for Clostridium difficile".

Authors:  P Pancholi; C Kelly; M Raczkowski; J M Balada-Llasat
Journal:  J Clin Microbiol       Date:  2013-05       Impact factor: 5.948

7.  Comparison of detection methods for Clostridium difficile.

Authors:  Monica Ianosi-Irimie; Dawn Morong; Catherine Dragoni; Stanley Schofield
Journal:  J Clin Microbiol       Date:  2013-05       Impact factor: 5.948

8.  Novel portable platform for molecular detection of toxigenic Clostridium difficile in faeces: a diagnostic accuracy study.

Authors:  J J Hirvonen; P Matero; C Siebert; J Kauppila; R Vuento; H Tuokko; S Boisset
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2016-12-17       Impact factor: 3.267

9.  Comparison of Illumigene, Simplexa, and AmpliVue Clostridium difficile molecular assays for diagnosis of C. difficile infection.

Authors:  E Deak; S A Miller; R M Humphries
Journal:  J Clin Microbiol       Date:  2013-12-18       Impact factor: 5.948

Review 10.  Clostridium difficile infection: epidemiology, diagnosis and understanding transmission.

Authors:  Jessica S H Martin; Tanya M Monaghan; Mark H Wilcox
Journal:  Nat Rev Gastroenterol Hepatol       Date:  2016-03-09       Impact factor: 46.802
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