Literature DB >> 17554497

13C- 13C NOESY spectra of a 480 kDa protein: solution NMR of ferritin.

Manolis Matzapetakis1, Paola Turano, Elizabeth C Theil, Ivano Bertini.   

Abstract

Molecular size has limited solution NMR analyses of proteins. We report (13)C-(13)C NOESY experiments on a 480 kDa protein, the multi-subunit ferritin nanocage with gated pores. By exploiting (13)C-resonance-specific chemical shifts and spin diffusion effects, we identified 75% of the amino acids, with intraresidue C-C connectivities between nuclei separated by 1-4 bonds. These results show the potential of (13)C-(13)C NOESY for solution studies of molecular assemblies >100 kDa.

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Year:  2007        PMID： 17554497     DOI： 10.1007/s10858-007-9163-9

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  22 in total

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Authors:  Dominique P Frueh; Zhen-Yu J Sun; David A Vosburg; Christopher T Walsh; Jeffrey C Hoch; Gerhard Wagner
Journal:  J Am Chem Soc       Date:  2006-05-03       Impact factor: 15.419

2.  NMR analysis of a 900K GroEL GroES complex.

Authors:  Jocelyne Fiaux; Eric B Bertelsen; Arthur L Horwich; Kurt Wüthrich
Journal:  Nature       Date:  2002-07-11       Impact factor: 49.962

3.  Solution NMR-derived global fold of a monomeric 82-kDa enzyme.

Authors:  Vitali Tugarinov; Wing-Yiu Choy; Vladislav Yu Orekhov; Lewis E Kay
Journal:  Proc Natl Acad Sci U S A       Date:  2005-01-06       Impact factor: 11.205

4.  (13)C-(13)C NOESY: a constructive use of (13)C-(13)C spin-diffusion.

Authors:  Ivano Bertini; Isabella C Felli; Rainer Kümmerle; Claudio Luchinat; Roberta Pierattelli
Journal:  J Biomol NMR       Date:  2004-11       Impact factor: 2.835

5.  Protonless NMR experiments for sequence-specific assignment of backbone nuclei in unfolded proteins.

Authors:  Wolfgang Bermel; Ivano Bertini; Isabella C Felli; Yong-Min Lee; Claudio Luchinat; Roberta Pierattelli
Journal:  J Am Chem Soc       Date:  2006-03-29       Impact factor: 15.419

6.  A protein structure from nuclear magnetic resonance data. lac repressor headpiece.

Authors:  R Kaptein; E R Zuiderweg; R M Scheek; R Boelens; W F van Gunsteren
Journal:  J Mol Biol       Date:  1985-03-05       Impact factor: 5.469

7.  NMR techniques used with very large biological macromolecules in solution.

Authors:  Gerhard Wider
Journal:  Methods Enzymol       Date:  2005       Impact factor: 1.600

8.  13C-13C NOESY: an attractive alternative for studying large macromolecules.

Authors:  Ivano Bertini; Isabella C Felli; Rainer Kümmerle; Detlef Moskau; Roberta Pierattelli
Journal:  J Am Chem Soc       Date:  2004-01-21       Impact factor: 15.419

9.  Uniform and residue-specific 15N-labeling of proteins on a highly deuterated background.

Authors:  Jocelyne Fiaux; Eric B Bertelsen; Arthur L Horwich; Kurt Wüthrich
Journal:  J Biomol NMR       Date:  2004-07       Impact factor: 2.835

10.  13C direct detection experiments on the paramagnetic oxidized monomeric copper, zinc superoxide dismutase.

Authors:  Wolfgang Bermel; Ivano Bertini; Isabella C Felli; Rainer Kümmerle; Roberta Pierattelli
Journal:  J Am Chem Soc       Date:  2003-12-31       Impact factor: 15.419
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  12 in total

1.  SedNMR: a web tool for optimizing sedimentation of macromolecular solutes for SSNMR.

Authors:  Lucio Ferella; Claudio Luchinat; Enrico Ravera; Antonio Rosato
Journal:  J Biomol NMR       Date:  2013-11-17       Impact factor: 2.835

2.  Solid-state NMR of proteins sedimented by ultracentrifugation.

Authors:  Ivano Bertini; Claudio Luchinat; Giacomo Parigi; Enrico Ravera; Bernd Reif; Paola Turano
Journal:  Proc Natl Acad Sci U S A       Date:  2011-06-13       Impact factor: 11.205

3.  Topical Developments in High-Field Dynamic Nuclear Polarization.

Authors:  Vladimir K Michaelis; Ta-Chung Ong; Matthew K Kiesewetter; Derik K Frantz; Joseph J Walish; Enrico Ravera; Claudio Luchinat; Timothy M Swager; Robert G Griffin
Journal:  Isr J Chem       Date:  2014-02-13       Impact factor: 3.333

4.  On the use of ultracentrifugal devices for sedimented solute NMR.

Authors:  Ivano Bertini; Frank Engelke; Leonardo Gonnelli; Benno Knott; Claudio Luchinat; David Osen; Enrico Ravera
Journal:  J Biomol NMR       Date:  2012-08-08       Impact factor: 2.835

5.  Coordinating subdomains of ferritin protein cages with catalysis and biomineralization viewed from the C4 cage axes.

Authors:  Elizabeth C Theil; Paola Turano; Veronica Ghini; Marco Allegrozzi; Caterina Bernacchioni
Journal:  J Biol Inorg Chem       Date:  2014-02-07       Impact factor: 3.358

6.  NMR reveals pathway for ferric mineral precursors to the central cavity of ferritin.

Authors:  Paola Turano; Daniela Lalli; Isabella C Felli; Elizabeth C Theil; Ivano Bertini
Journal:  Proc Natl Acad Sci U S A       Date:  2009-12-16       Impact factor: 11.205

7.  Dynamic nuclear polarization of sedimented solutes.

Authors:  Enrico Ravera; Björn Corzilius; Vladimir K Michaelis; Camilla Rosa; Robert G Griffin; Claudio Luchinat; Ivano Bertini
Journal:  J Am Chem Soc       Date:  2013-01-25       Impact factor: 15.419

8.  Relaxation-optimised Hartmann-Hahn transfer using a specifically Tailored MOCCA-XY16 mixing sequence for carbonyl-carbonyl correlation spectroscopy in 13C direct detection NMR experiments.

Authors:  Isabella C Felli; Roberta Pierattelli; Steffen J Glaser; Burkhard Luy
Journal:  J Biomol NMR       Date:  2009-02-18       Impact factor: 2.835

Review 9.  Ferritin: the protein nanocage and iron biomineral in health and in disease.

Authors:  Elizabeth C Theil
Journal:  Inorg Chem       Date:  2013-10-08       Impact factor: 5.165

Review 10.  Facing and Overcoming Sensitivity Challenges in Biomolecular NMR Spectroscopy.

Authors:  Jan-Henrik Ardenkjaer-Larsen; Gregory S Boebinger; Arnaud Comment; Simon Duckett; Arthur S Edison; Frank Engelke; Christian Griesinger; Robert G Griffin; Christian Hilty; Hidaeki Maeda; Giacomo Parigi; Thomas Prisner; Enrico Ravera; Jan van Bentum; Shimon Vega; Andrew Webb; Claudio Luchinat; Harald Schwalbe; Lucio Frydman
Journal:  Angew Chem Int Ed Engl       Date:  2015-07-01       Impact factor: 15.336
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