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Literature DB >> 16432517

Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR).

David S Burz¹, Kaushik Dutta, David Cowburn, Alexander Shekhtman.

Abstract

We describe a high-throughput in-cell nuclear magnetic resonance (NMR)-based method for mapping the structural changes that accompany protein-protein interactions (STINT-NMR). The method entails sequentially expressing two (or more) proteins within a single bacterial cell in a time-controlled manner and monitoring the protein interactions using in-cell NMR spectroscopy. The resulting spectra provide a complete titration of the interaction and define structural details of the interacting surfaces at atomic resolution.

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Year: 2006 PMID： 16432517 PMCID： PMC4447212 DOI： 10.1038/nmeth851

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

15 in total

1. Integrative database analysis in structural genomics.

Authors: M Gerstein
Journal: Nat Struct Biol Date: 2000-11

Review 2. In-cell NMR spectroscopy.

Authors: Z Serber; V Dötsch
Journal: Biochemistry Date: 2001-12-04 Impact factor: 3.162

3. Structure and ubiquitin binding of the ubiquitin-interacting motif.

Authors: Robert D Fisher; Bin Wang; Steven L Alam; Daniel S Higginson; Howard Robinson; Wesley I Sundquist; Christopher P Hill
Journal: J Biol Chem Date: 2003-05-14 Impact factor: 5.157

Review 4. Ubiquitin: structures, functions, mechanisms.

Authors: Cecile M Pickart; Michael J Eddins
Journal: Biochim Biophys Acta Date: 2004-11-29

Review 5. Molecular machines for protein degradation.

Authors: Michael Groll; Matthias Bochtler; Hans Brandstetter; Tim Clausen; Robert Huber
Journal: Chembiochem Date: 2005-02 Impact factor: 3.164

6. Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.

Authors: Sara Züger; Hideo Iwai
Journal: Nat Biotechnol Date: 2005-05-22 Impact factor: 54.908

7. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.

Authors: R Lutz; H Bujard
Journal: Nucleic Acids Res Date: 1997-03-15 Impact factor: 16.971

8. STAM proteins bind ubiquitinated proteins on the early endosome via the VHS domain and ubiquitin-interacting motif.

Authors: Emi Mizuno; Kensuke Kawahata; Masaki Kato; Naomi Kitamura; Masayuki Komada
Journal: Mol Biol Cell Date: 2003-06-13 Impact factor: 4.138

9. A ubiquitin-interacting motif from Hrs binds to and occludes the ubiquitin surface necessary for polyubiquitination in monoubiquitinated proteins.

Authors: Alexander Shekhtman; David Cowburn
Journal: Biochem Biophys Res Commun Date: 2002-09-06 Impact factor: 3.575

10. STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes.

Authors: Kristi G Bache; Camilla Raiborg; Anja Mehlum; Harald Stenmark
Journal: J Biol Chem Date: 2003-01-27 Impact factor: 5.157

55 in total

1. NMR protein structure determination in living E. coli cells using nonlinear sampling.

Authors: Teppei Ikeya; Atsuko Sasaki; Daisuke Sakakibara; Yoshiki Shigemitsu; Junpei Hamatsu; Tomomi Hanashima; Masaki Mishima; Masatoshi Yoshimasu; Nobuhiro Hayashi; Tsutomu Mikawa; Daniel Nietlispach; Markus Wälchli; Brian O Smith; Masahiro Shirakawa; Peter Güntert; Yutaka Ito
Journal: Nat Protoc Date: 2010-05-13 Impact factor: 13.491

Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR).

1. Integrative database analysis in structural genomics.

Review 2. In-cell NMR spectroscopy.

3. Structure and ubiquitin binding of the ubiquitin-interacting motif.

Review 4. Ubiquitin: structures, functions, mechanisms.

Review 5. Molecular machines for protein degradation.

6. Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.

7. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.

8. STAM proteins bind ubiquitinated proteins on the early endosome via the VHS domain and ubiquitin-interacting motif.

9. A ubiquitin-interacting motif from Hrs binds to and occludes the ubiquitin surface necessary for polyubiquitination in monoubiquitinated proteins.

10. STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes.

1. NMR protein structure determination in living E. coli cells using nonlinear sampling.

2. Suppression of phospholipid biosynthesis by cerulenin in the condensed Single-Protein-Production (cSPP) system.

Review 3. Biological synthesis of circular polypeptides.

Review 4. A Unique Tool for Cellular Structural Biology: In-cell NMR.

5. Ribosome Mediated Quinary Interactions Modulate In-Cell Protein Activities.

6. MetJ repressor interactions with DNA probed by in-cell NMR.

Review 7. Site-specific labeling of proteins with NMR-active unnatural amino acids.

8. Controlling and quantifying protein concentration in Escherichia coli.

Review 9. In-Cell NMR Spectroscopy of Intrinsically Disordered Proteins.

10. Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.