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Literature DB >> 10872324

Purification and characterization of Xenopus laevis topoisomerase II.

P Benedetti¹, M I Baldi, E Mattoccia, G P Tocchini-Valentini.

Abstract

We have purified to apparent homogeneity a type II DNA topoisomerase from Xenopus laevis oocyte nuclei (germinal vesicles, or GV). The most pure preparations contain a single polypeptide of 175,000 daltons as determined by SDS-gel electrophoresis. The enzyme changes the linking number of DNA circles in steps of two and reversibly knots or catenates DNA rings. No gyrase activity is detectable and ATP is required.

Entities: Chemical

Mesh：

Substances：

Year: 1983 PMID： 10872324 PMCID： PMC555276 DOI： 10.1002/j.1460-2075.1983.tb01585.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

25 in total

1. A sign inversion mechanism for enzymatic supercoiling of DNA.

Authors: P O Brown; N R Cozzarelli
Journal: Science Date: 1979-11-30 Impact factor: 47.728

2. DNA gyrase: an enzyme that introduces superhelical turns into DNA.

Authors: M Gellert; K Mizuuchi; M H O'Dea; H A Nash
Journal: Proc Natl Acad Sci U S A Date: 1976-11 Impact factor: 11.205

3. DNA-relaxing activity and endonuclease activity in Xenopus laevis oocytes.

Authors: E Mattoccia; D G Attardi; G P Tocchini-Valentini
Journal: Proc Natl Acad Sci U S A Date: 1976-12 Impact factor: 11.205

4. DNA gyrase action involves the introduction of transient double-strand breaks into DNA.

Authors: K Mizuuchi; L M Fisher; M H O'Dea; M Gellert
Journal: Proc Natl Acad Sci U S A Date: 1980-04 Impact factor: 11.205

Review 5. Proteins that affect DNA conformation.

Authors: J J Champoux
Journal: Annu Rev Biochem Date: 1978 Impact factor: 23.643

6. Energy coupling in DNA gyrase and the mechanism of action of novobiocin.

Authors: A Sugino; N P Higgins; P O Brown; C L Peebles; N R Cozzarelli
Journal: Proc Natl Acad Sci U S A Date: 1978-10 Impact factor: 11.205

7. Type II DNA topoisomerases: enzymes that can unknot a topologically knotted DNA molecule via a reversible double-strand break.

Authors: L F Liu; C C Liu; B M Alberts
Journal: Cell Date: 1980-03 Impact factor: 41.582

8. Formation and resolution of DNA catenanes by DNA gyrase.

Authors: K N Kreuzer; N R Cozzarelli
Journal: Cell Date: 1980-05 Impact factor: 41.582

Review 9. DNA gyrase and the supercoiling of DNA.

Authors: N R Cozzarelli
Journal: Science Date: 1980-02-29 Impact factor: 47.728

10. Separation of RNA transcription and processing activities from X. laevis germinal vesicles.

Authors: E Mattoccia; M I Baldi; G Carrara; P Fruscoloni; P Benedetti; G P Tocchini-Valentini
Journal: Cell Date: 1979-11 Impact factor: 41.582

11 in total

1. Cloning and characterization of a cDNA encoding topoisomerase II in pea and analysis of its expression in relation to cell proliferation.

Authors: M K Reddy; S Nair; K K Tewari; Y Mudgil; B S Yadav; S K Sopory
Journal: Plant Mol Biol Date: 1999-09 Impact factor: 4.076

2. Differential compartmentalization of plasmid DNA microinjected into Xenopus laevis embryos relates to replication efficiency.

Authors: N J Marini; R M Benbow
Journal: Mol Cell Biol Date: 1991-01 Impact factor: 4.272

8. The nucleotide sequence of the fission yeast DNA topoisomerase II gene: structural and functional relationships to other DNA topoisomerases.

Authors: T Uemura; K Morikawa; M Yanagida
Journal: EMBO J Date: 1986-09 Impact factor: 11.598

9. Assembly of spaced chromatin involvement of ATP and DNA topoisomerase activity.

Authors: G Almouzni; M Méchali
Journal: EMBO J Date: 1988-12-20 Impact factor: 11.598

10. The positive transcription factor of the 5S RNA gene proteolyses during direct exchange between 5S DNA sites.

Authors: E B Kmiec; A Worcel
Journal: J Cell Biol Date: 1986-09 Impact factor: 10.539