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Literature DB >> 519751

Separation of RNA transcription and processing activities from X. laevis germinal vesicles.

E Mattoccia, M I Baldi, G Carrara, P Fruscoloni, P Benedetti, G P Tocchini-Valentini.

Abstract

A procedure suitable for en masse preparation of germinal vesicles (GV) from X.laevis oocytes (Scalenghe et al., 1978) has been adapted for studies of transcription. Extracts from GV contain activities for transcription of tRNA genes and for processing the transcription product. The two activities have been separated by column chromatography. One fraction allows synthesis of tRNA precursor molecules in the presence of X.laevis RNA polymerase III. Another fraction contains the activity that cuts and splices those precursors which contain an intervening sequence. Transcription occurs faithfully on linear DNA fragments.

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Year: 1979 PMID： 519751 DOI： 10.1016/0092-8674(79)90119-3

Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582

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Cited

11 in total

1. Splicing of mRNA mediated by tRNA sequences in mouse cells.

Authors: Michela Zamboni; Daniela Scarabino; Glauco P Tocchini-Valentini
Journal: RNA Date: 2009-10-22 Impact factor: 4.942

2. Identification of multiple RNases in Xenopus laevis oocytes and their possible role in tRNA processing.

Authors: A Solari; M P Deutscher
Journal: Mol Cell Biol Date: 1983-10 Impact factor: 4.272

3. The nucleotide sequence of glutamate tRNA4 of Drosophila melanogaster.

Authors: M Altwegg; E Kubli
Journal: Nucleic Acids Res Date: 1980-01-25 Impact factor: 16.971

4. Ribonuclease "XlaI," an activity from Xenopus laevis oocytes that excises intervening sequences from yeast transfer ribonucleic acid precursors.

Authors: A Otsuka; A de Paolis; G P Tocchini-Valentini
Journal: Mol Cell Biol Date: 1981-03 Impact factor: 4.272

5. Transcription and processing of a yeast tRNA gene containing a modified intervening sequence.

Authors: J D Johnson; R Ogden; P Johnson; J Abelson; P Dembeck; K Itakura
Journal: Proc Natl Acad Sci U S A Date: 1980-05 Impact factor: 11.205

6. Selective in vitro transcription of one of the two Alu family repeats present in the 5' flanking region of the human epsilon-globin gene.

Authors: G Di Segni; G Carrara; G R Tocchini-Valentini; C C Shoulders; F E Baralle
Journal: Nucleic Acids Res Date: 1981-12-21 Impact factor: 16.971

Separation of RNA transcription and processing activities from X. laevis germinal vesicles.

1. Splicing of mRNA mediated by tRNA sequences in mouse cells.

2. Identification of multiple RNases in Xenopus laevis oocytes and their possible role in tRNA processing.

3. The nucleotide sequence of glutamate tRNA4 of Drosophila melanogaster.

4. Ribonuclease "XlaI," an activity from Xenopus laevis oocytes that excises intervening sequences from yeast transfer ribonucleic acid precursors.

5. Transcription and processing of a yeast tRNA gene containing a modified intervening sequence.

6. Selective in vitro transcription of one of the two Alu family repeats present in the 5' flanking region of the human epsilon-globin gene.

7. A yeast transcription system for the 5S rRNA gene.

8. A pre-tRNA carrying intron features typical of Archaea is spliced in yeast.

9. Purification and characterization of Xenopus laevis topoisomerase II.

10. Wheat germ splicing endonuclease is highly specific for plant pre-tRNAs.