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Literature DB >> 9740124

Circular mRNA can direct translation of extremely long repeating-sequence proteins in vivo.

Abstract

Many proteins with unusual structural properties are comprised of multiple repeating amino acid sequences and are often fractious to expression in recombinant systems. To facilitate recombinant production of such proteins for structural and engineering studies, we have produced circular messenger RNAs with infinite open reading frames. We show that a circular mRNA containing a simple green fluorescent protein (GFP) open reading frame can direct GFP expression in Escherichia coli. A circular mRNA with an infinite GFP open reading frame produces extremely long protein chains, proving that bacterial ribosomes can internally initiate and repeatedly transit a circular mRNA. Only the monomeric forms of GFP produced from circular mRNA are fluorescent. Analysis of the translation initiation region shows that multiple sequences contribute to maximal translation from circular mRNA. This technology provides a unique means of producing a very long repeating-sequence protein, and may open the way for development of proteinaceous materials with novel properties.

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Year: 1998 PMID： 9740124 PMCID： PMC1369681 DOI： 10.1017/s135583829898061x

Source DB: PubMed Journal: RNA ISSN： 1355-8382 Impact factor: 4.942

37 in total

1. Protein-coding introns from the 23S rRNA-encoding gene form stable circles in the hyperthermophilic archaeon Pyrobaculum organotrophum.

Authors: J Z Dalgaard; R A Garrett
Journal: Gene Date: 1992-11-02 Impact factor: 3.688

2. Scrambled exons.

Authors: J M Nigro; K R Cho; E R Fearon; S E Kern; J M Ruppert; J D Oliner; K W Kinzler; B Vogelstein
Journal: Cell Date: 1991-02-08 Impact factor: 41.582

3. Initiation of protein synthesis by the eukaryotic translational apparatus on circular RNAs.

Authors: C Y Chen; P Sarnow
Journal: Science Date: 1995-04-21 Impact factor: 47.728

4. Synthesis of circular RNA in bacteria and yeast using RNA cyclase ribozymes derived from a group I intron of phage T4.

Authors: E Ford; M Ares
Journal: Proc Natl Acad Sci U S A Date: 1994-04-12 Impact factor: 11.205

5. A site-specific endonuclease encoded by a typical archaeal intron.

Authors: J Z Dalgaard; R A Garrett; M Belfort
Journal: Proc Natl Acad Sci U S A Date: 1993-06-15 Impact factor: 11.205

6. Green fluorescent protein as a marker for gene expression.

Authors: M Chalfie; Y Tu; G Euskirchen; W W Ward; D C Prasher
Journal: Science Date: 1994-02-11 Impact factor: 47.728

7. The use of a tRNA as a transcriptional reporter: the T7 late promoter is extremely efficient in Escherichia coli but its transcripts are poorly expressed.

Authors: P J Lopez; I Iost; M Dreyfus
Journal: Nucleic Acids Res Date: 1994-04-11 Impact factor: 16.971

8. Splicing with inverted order of exons occurs proximal to large introns.

Authors: C Cocquerelle; P Daubersies; M A Majérus; J P Kerckaert; B Bailleul
Journal: EMBO J Date: 1992-03 Impact factor: 11.598

9. The identity of the base following the stop codon determines the efficiency of in vivo translational termination in Escherichia coli.

Authors: E S Poole; C M Brown; W P Tate
Journal: EMBO J Date: 1995-01-03 Impact factor: 11.598

10. Circular transcripts of the testis-determining gene Sry in adult mouse testis.

Authors: B Capel; A Swain; S Nicolis; A Hacker; M Walter; P Koopman; P Goodfellow; R Lovell-Badge
Journal: Cell Date: 1993-06-04 Impact factor: 41.582

51 in total

Review 1. Insights into circular RNA biology.

Authors: Karoline K Ebbesen; Thomas B Hansen; Jørgen Kjems
Journal: RNA Biol Date: 2016-12-16 Impact factor: 4.652

Circular mRNA can direct translation of extremely long repeating-sequence proteins in vivo.

1. Protein-coding introns from the 23S rRNA-encoding gene form stable circles in the hyperthermophilic archaeon Pyrobaculum organotrophum.

2. Scrambled exons.

3. Initiation of protein synthesis by the eukaryotic translational apparatus on circular RNAs.

4. Synthesis of circular RNA in bacteria and yeast using RNA cyclase ribozymes derived from a group I intron of phage T4.

5. A site-specific endonuclease encoded by a typical archaeal intron.

6. Green fluorescent protein as a marker for gene expression.

7. The use of a tRNA as a transcriptional reporter: the T7 late promoter is extremely efficient in Escherichia coli but its transcripts are poorly expressed.

8. Splicing with inverted order of exons occurs proximal to large introns.

9. The identity of the base following the stop codon determines the efficiency of in vivo translational termination in Escherichia coli.

10. Circular transcripts of the testis-determining gene Sry in adult mouse testis.

Review 1. Insights into circular RNA biology.

Review 2. Circular RNAs: analysis, expression and potential functions.

3. Sensing Self and Foreign Circular RNAs by Intron Identity.

4. Circular RNAs in leukemia.

Review 5. The emerging role of circular RNAs in gastric cancer.

Review 6. Circular RNAs and hereditary bone diseases.

Review 7. Emerging roles and context of circular RNAs.

Review 8. Circular RNAs open a new chapter in cardiovascular biology.

Review 9. The design and synthesis of circular RNAs.

10. Rolling-translated EGFR variants sustain EGFR signaling and promote glioblastoma tumorigenicity.