| Literature DB >> 9224596 |
M L Collins1, B Irvine, D Tyner, E Fine, C Zayati, C Chang, T Horn, D Ahle, J Detmer, L P Shen, J Kolberg, S Bushnell, M S Urdea, D D Ho.
Abstract
The branched DNA hybridization assay has been improved by the inclusion of the novel nucleotides, isoC and isoG, in the amplification sequences to prevent non-specific hybridization. The novel isoC, isoG-containing amplification sequences have no detectable interaction with any natural DNA sequence. The control of non-specific hybridization in turn permits increased signal amplification. Addition of a 14 site preamplifier was found to increase the signal/noise ratio 8-fold. A set of 74 oligonucleotide probes was designed to the consensus HIV POL sequence. The detection limit of this new HIV branched DNA amplifier assay was approximately 50 molecules/ml. The assay was used to measure viral load in 87 plasma samples of HIV- infected patients on triple drug therapy whose RNA titers were <500 molecules/ml. In all 11 patients viral load eventually declined to below the detection limit with the new assay.Entities:
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Year: 1997 PMID： 9224596 PMCID： PMC146852 DOI： 10.1093/nar/25.15.2979
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971