Literature DB >> 8616271

Correlation of the guanosine exchangeable and nonexchangeable base protons in 13C-/15N-labeled RNA with an HNC-TOCSY-CH experiment.

J P Simorre1, G R Zimmermann, L Mueller, A Pardi.   

Abstract

A triple resonance HNC-TOCSY-CH experiment is described for correlating the guanosine imino proton and H8 resonances in 13C-/15N-labeled RNAs. Sequential assignment of the exchangeable imino protons in Watson-Crick base pairs is generally made independently of the assignment of the nonexchangeable base protons. This H(NC)-TOCSY-(C)H experiment makes it possible to unambiguously link the assignment of the guanosine H8 resonances with sequential assignment of the guanosine imino proton resonances. 2D H(NC)-TOCSY-(C)H spectra are presented for two isotopically labeled RNAs, a 30-nucleotide lead-dependent ribozyme known as the leadzyme, and a 48-nucleotide hammerhead ribozyme-RNA substrate complex. The results obtained on these two RNAs demonstrate that this HNC-TOCSY-CH experiment is an important tool for resonance assignment of isotopically labeled RNAs.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8616271     DOI： 10.1007/bf00203825

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  11 in total

1.  A small metalloribozyme with a two-step mechanism.

Authors:  T Pan; O C Uhlenbeck
Journal:  Nature       Date:  1992-08-13       Impact factor: 49.962

2.  Structural features that give rise to the unusual stability of RNA hairpins containing GNRA loops.

Authors:  H A Heus; A Pardi
Journal:  Science       Date:  1991-07-12       Impact factor: 47.728

3.  A small catalytic oligoribonucleotide.

Authors:  O C Uhlenbeck
Journal:  Nature       Date:  1987 Aug 13-19       Impact factor: 49.962

4.  Multidimensional heteronuclear NMR experiments for structure determination of isotopically labeled RNA.

Authors:  A Pardi
Journal:  Methods Enzymol       Date:  1995       Impact factor: 1.600

Review 5.  Pulsed-field gradients: theory and practice.

Authors:  J Keeler; R T Clowes; A L Davis; E D Laue
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

6.  Preparation of 13C and 15N labelled RNAs for heteronuclear multi-dimensional NMR studies.

Authors:  E P Nikonowicz; A Sirr; P Legault; F M Jucker; L M Baer; A Pardi
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

7.  Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA.

Authors:  R T Batey; M Inada; E Kujawinski; J D Puglisi; J R Williamson
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

8.  A general method for assigning NMR spectra of denatured proteins using 3D HC(CO)NH-TOCSY triple resonance experiments.

Authors:  T M Logan; E T Olejniczak; R X Xu; S W Fesik
Journal:  J Biomol NMR       Date:  1993-03       Impact factor: 2.835

9.  Triple resonance HNCCCH experiments for correlating exchangeable and nonexchangeable cytidine and uridine base protons in RNA.

Authors:  J P Simorre; G R Zimmermann; A Pardi; B T Farmer; L Mueller
Journal:  J Biomol NMR       Date:  1995-12       Impact factor: 2.835

10.  Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.

Authors:  M Piotto; V Saudek; V Sklenár
Journal:  J Biomol NMR       Date:  1992-11       Impact factor: 2.835
View more
  14 in total

1.  Long-range imino proton-13C J-couplings and the through-bond correlation of imino and non-exchangeable protons in unlabeled DNA.

Authors:  A T Phan
Journal:  J Biomol NMR       Date:  2000-02       Impact factor: 2.835

2.  Redor in IS1S2 systems.

Authors:  J Leppert; B Heise; R Ramachandran
Journal:  J Biomol NMR       Date:  2000-10       Impact factor: 2.835

3.  Assignment methodology for larger RNA oligonucleotides: application to an ATP-binding RNA aptamer.

Authors:  T Dieckmann; J Feigon
Journal:  J Biomol NMR       Date:  1997-04       Impact factor: 2.835

4.  Transverse relaxation optimized triple-resonance NMR experiments for nucleic acids.

Authors:  R Fiala; J Czernek; V Sklenár
Journal:  J Biomol NMR       Date:  2000-04       Impact factor: 2.835

5.  Simple, efficient protocol for enzymatic synthesis of uniformly 13C, 15N-labeled DNA for heteronuclear NMR studies.

Authors:  J E Masse; P Bortmann; T Dieckmann; J Feigon
Journal:  Nucleic Acids Res       Date:  1998-06-01       Impact factor: 16.971

6.  Resolution-enhanced base-type-edited HCN experiment for RNA.

Authors:  Hélène Van Melckebeke; Arthur Pardi; Jérôme Boisbouvier; Jean-Pierre Simorre; Bernhard Brutscher
Journal:  J Biomol NMR       Date:  2005-08       Impact factor: 2.835

7.  A TROSY relayed HCCH-COSY experiment for correlating adenine H2/H8 resonances in uniformly 13C-labeled RNA molecules.

Authors:  B Simon; K Zanier; M Sattler
Journal:  J Biomol NMR       Date:  2001-06       Impact factor: 2.835

8.  RNA binding and chaperone activity of the E. coli cold-shock protein CspA.

Authors:  Enrico Rennella; Tomáš Sára; Michael Juen; Christoph Wunderlich; Lionel Imbert; Zsofia Solyom; Adrien Favier; Isabel Ayala; Katharina Weinhäupl; Paul Schanda; Robert Konrat; Christoph Kreutz; Bernhard Brutscher
Journal:  Nucleic Acids Res       Date:  2017-04-20       Impact factor: 16.971

9.  Experiments for correlating quaternary carbons in RNA bases.

Authors:  Radovan Fiala; Markéta L Munzarová; Vladimír Sklenár
Journal:  J Biomol NMR       Date:  2004-08       Impact factor: 2.835

10.  Triple resonance experiments for the simultaneous correlation of H6/H5 and exchangeable protons of pyrimidine nucleotides in 13C,15N-labeled RNA applicable to larger RNA molecules.

Authors:  Jens Wöhnert; Matthias Görlach; Harald Schwalbe
Journal:  J Biomol NMR       Date:  2003-05       Impact factor: 2.835
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.