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Literature DB >> 8563469

Triple resonance HNCCCH experiments for correlating exchangeable and nonexchangeable cytidine and uridine base protons in RNA.

J P Simorre¹, G R Zimmermann, A Pardi, B T Farmer, L Mueller.

Abstract

A set of triple resonance experiments is presented, providing through-bond H2N/HN to H6 connectivities in uridines and cytidines in 13C-/15N-labeled RNAs. These connectivities provide an important link between the sequential assignment pathways for the exchangeable and nonexchangeable proton resonances in nucleic acids. Both 2D and pseudo-3D HNCCCH experiments were applied to a 30-nucleotide lead-dependent ribozyme, known as the leadzyme. The HN to H6 connectivities for three uridines in the leadzyme were identified from one 2D H(NCCC)H experiment, and the H2N to H6 connectivities were identified for seven of the eight cytidines from the combination of a 2D H(NCCC)H and a pseudo-3D H(NCC)CH experiment.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1995 PMID： 8563469 DOI： 10.1007/bf00197641

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

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9. Unambiguous through-bond sugar-to-base correlations for purines in 13C,15N-labeled nucleic acids: the HsCsNb,HsCs(N)bCb, and HbNbCb experiments.

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10. Sequential backbone assignment of uniformly 13C-labeled RNAs by a two-dimensional P(CC)H-TOCSY triple resonance NMR experiment.

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23 in total

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2. Long-range imino proton-13C J-couplings and the through-bond correlation of imino and non-exchangeable protons in unlabeled DNA.

Authors: A T Phan
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Triple resonance HNCCCH experiments for correlating exchangeable and nonexchangeable cytidine and uridine base protons in RNA.

1. A small metalloribozyme with a two-step mechanism.

2. Use of a water flip-back pulse in the homonuclear NOESY experiment.

3. Multidimensional heteronuclear NMR experiments for structure determination of isotopically labeled RNA.

4. An efficient procedure for assignment of the proton, carbon and nitrogen resonances in 13C/15N labeled nucleic acids.

5. Two- and three-dimensional HCN experiments for correlating base and sugar resonances in 15N,13C-labeled RNA oligonucleotides.

6. Improved measurement of 13C, 31P J coupling constants in isotopically labeled RNA.

7. NMR of enzymatically synthesized uniformly 13C15N-labeled DNA oligonucleotides.

8. Assignment of NMR spectra of proteins using triple-resonance two-dimensional experiments.

9. Unambiguous through-bond sugar-to-base correlations for purines in 13C,15N-labeled nucleic acids: the HsCsNb,HsCs(N)bCb, and HbNbCb experiments.

10. Sequential backbone assignment of uniformly 13C-labeled RNAs by a two-dimensional P(CC)H-TOCSY triple resonance NMR experiment.

1. Assignment of cytosine N3 resonances in nucleic acids via intrabase three-bond coupling to amino protons.

2. Long-range imino proton-13C J-couplings and the through-bond correlation of imino and non-exchangeable protons in unlabeled DNA.

3. Redor in IS1S2 systems.

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7. Transverse relaxation optimized triple-resonance NMR experiments for nucleic acids.

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