Literature DB >> 7972114

Three-dimensional structure of extended chromatin fibers as revealed by tapping-mode scanning force microscopy.

S H Leuba1, G Yang, C Robert, B Samori, K van Holde, J Zlatanova, C Bustamante.   

Abstract

Unfixed chicken erythrocyte chromatin fibers in very low salt have been imaged with a scanning force microscope operating in the tapping mode in air at ambient humidity. These images reveal a three-dimensional organization of the fibers. The planar "zig-zag" conformation is rare, and extended "beads-on-a-string" fibers are seen only in chromatin depleted of histones H1 and H5. Glutaraldehyde fixation reveals very similar structures. Fibers fixed in 10 mM salt appear somewhat more compacted. These results, when compared with modeling studies, suggest that chromatin fibers may exist as irregular three-dimensional arrays of nucleosomes even at low ionic strength.

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Year:  1994        PMID： 7972114      PMCID： PMC45283          DOI： 10.1073/pnas.91.24.11621

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  Atomic force microscope.

Authors: 
Journal:  Phys Rev Lett       Date:  1986-03-03       Impact factor: 9.161

2.  Salt-induced release of DNA from nucleosome core particles.

Authors:  T D Yager; C T McMurray; K E van Holde
Journal:  Biochemistry       Date:  1989-03-07       Impact factor: 3.162

Review 3.  Flexibility of DNA.

Authors:  P J Hagerman
Journal:  Annu Rev Biophys Biophys Chem       Date:  1988

4.  Accessibility of the globular domain of histones H1 and H5 to antibodies upon folding of chromatin.

Authors:  V R Russanova; S I Dimitrov; V L Makarov; I G Pashev
Journal:  Eur J Biochem       Date:  1987-09-01

5.  Spheroid chromatin units (v bodies).

Authors:  A L Olins; D E Olins
Journal:  Science       Date:  1974-01-25       Impact factor: 47.728

6.  A chromatin folding model that incorporates linker variability generates fibers resembling the native structures.

Authors:  C L Woodcock; S A Grigoryev; R A Horowitz; N Whitaker
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-01       Impact factor: 11.205

7.  Atomic force microscope measurements of nucleosome cores assembled along defined DNA sequences.

Authors:  M J Allen; X F Dong; T E O'Neill; P Yau; S C Kowalczykowski; J Gatewood; R Balhorn; E M Bradbury
Journal:  Biochemistry       Date:  1993-08-24       Impact factor: 3.162

8.  Unravelled nucleosomes, nucleosome beads and higher order structures of chromatin: influence of non-histone components and histone H1.

Authors:  F Thoma; T Koller
Journal:  J Mol Biol       Date:  1981-07-15       Impact factor: 5.469

9.  On the location of histones H1 and H5 in the chromatin fiber. Studies with immobilized trypsin and chymotrypsin.

Authors:  S H Leuba; J Zlatanova; K van Holde
Journal:  J Mol Biol       Date:  1993-02-20       Impact factor: 5.469

10.  Structure of nucleosomes and organization of internucleosomal DNA in chromatin.

Authors:  S G Bavykin; S I Usachenko; A O Zalensky; A D Mirzabekov
Journal:  J Mol Biol       Date:  1990-04-05       Impact factor: 5.469
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  66 in total

1.  DNA folding: structural and mechanical properties of the two-angle model for chromatin.

Authors:  H Schiessel; W M Gelbart; R Bruinsma
Journal:  Biophys J       Date:  2001-04       Impact factor: 4.033

2.  Pulling a single chromatin fiber reveals the forces that maintain its higher-order structure.

Authors:  Y Cui; C Bustamante
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-04       Impact factor: 11.205

3.  Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy.

Authors:  B Ladoux; J P Quivy; P Doyle; O du Roure; G Almouzni; J L Viovy
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-19       Impact factor: 11.205

4.  DNase I digestion reveals alternating asymmetrical protection of the nucleosome by the higher order chromatin structure.

Authors:  D Z Staynov
Journal:  Nucleic Acids Res       Date:  2000-08-15       Impact factor: 16.971

5.  Structure and stability of the lamin A tail domain and HGPS mutant.

Authors:  Zhao Qin; Agnieszka Kalinowski; Kris Noel Dahl; Markus J Buehler
Journal:  J Struct Biol       Date:  2011-05-24       Impact factor: 2.867

Review 6.  Optical tweezers stretching of chromatin.

Authors:  Lisa H Pope; Martin L Bennink; Jan Greve
Journal:  J Muscle Res Cell Motil       Date:  2002       Impact factor: 2.698

Review 7.  Stretching and imaging single DNA molecules and chromatin.

Authors:  Jordanka Zlatanova; Sanford H Leuba
Journal:  J Muscle Res Cell Motil       Date:  2002       Impact factor: 2.698

8.  Assembly of single chromatin fibers depends on the tension in the DNA molecule: magnetic tweezers study.

Authors:  Sanford H Leuba; Mikhail A Karymov; Miroslav Tomschik; Ravi Ramjit; Paul Smith; Jordanka Zlatanova
Journal:  Proc Natl Acad Sci U S A       Date:  2003-01-09       Impact factor: 11.205

9.  Molecular modeling of the chromatosome particle.

Authors:  M M Srinivas Bharath; Nagasuma R Chandra; M R S Rao
Journal:  Nucleic Acids Res       Date:  2003-07-15       Impact factor: 16.971

10.  Generic features of tertiary chromatin structure as detected in natural chromosomes.

Authors:  Waltraud G Müller; Dietmar Rieder; Gregor Kreth; Christoph Cremer; Zlatko Trajanoski; James G McNally
Journal:  Mol Cell Biol       Date:  2004-11       Impact factor: 4.272
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