Literature DB >> 12522259

Assembly of single chromatin fibers depends on the tension in the DNA molecule: magnetic tweezers study.

Sanford H Leuba1, Mikhail A Karymov, Miroslav Tomschik, Ravi Ramjit, Paul Smith, Jordanka Zlatanova.   

Abstract

We have used magnetic tweezers to study in real time chaperone-mediated chromatin assembly/disassembly at the level of single chromatin fibers. We find a strong dependence of the rate of assembly on the exerted force, with strong inhibition of assembly at forces exceeding 10 pN. During assembly, and especially at higher forces, occasional abrupt increases in the length of the fiber were observed, giving a clear indication of reversibility of the assembly process. This result is a clear demonstration of the dynamic equilibrium between nucleosome assembly and disassembly at the single chromatin fiber level.

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Year:  2003        PMID： 12522259      PMCID： PMC141023          DOI： 10.1073/pnas.0136890100

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy.

Authors:  B Ladoux; J P Quivy; P Doyle; O du Roure; G Almouzni; J L Viovy
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-19       Impact factor: 11.205

2.  DNA methylation-dependent chromatin fiber compaction in vivo and in vitro: requirement for linker histone.

Authors:  M A Karymov; M Tomschik; S H Leuba; P Caiafa; J Zlatanova
Journal:  FASEB J       Date:  2001-12       Impact factor: 5.191

3.  Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA.

Authors:  Brent D Brower-Toland; Corey L Smith; Richard C Yeh; John T Lis; Craig L Peterson; Michelle D Wang
Journal:  Proc Natl Acad Sci U S A       Date:  2002-02-19       Impact factor: 11.205

4.  Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers.

Authors:  M L Bennink; S H Leuba; G H Leno; J Zlatanova; B G de Grooth; J Greve
Journal:  Nat Struct Biol       Date:  2001-07

5.  Functional analysis of nucleosome assembly protein, NAP-1. The negatively charged COOH-terminal region is not necessary for the intrinsic assembly activity.

Authors:  T Fujii-Nakata; Y Ishimi; A Okuda; A Kikuchi
Journal:  J Biol Chem       Date:  1992-10-15       Impact factor: 5.157

6.  Atomic force microscope.

Authors: 
Journal:  Phys Rev Lett       Date:  1986-03-03       Impact factor: 9.161

7.  Driving proteins off DNA using applied tension.

Authors:  J F Marko; E D Siggia
Journal:  Biophys J       Date:  1997-10       Impact factor: 4.033

8.  Crystal structure of the nucleosome core particle at 2.8 A resolution.

Authors:  K Luger; A W Mäder; R K Richmond; D F Sargent; T J Richmond
Journal:  Nature       Date:  1997-09-18       Impact factor: 49.962

9.  Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure.

Authors:  R T Simpson; F Thoma; J M Brubaker
Journal:  Cell       Date:  1985-10       Impact factor: 41.582

10.  Multistep chromatin assembly on supercoiled plasmid DNA by nucleosome assembly protein-1 and ATP-utilizing chromatin assembly and remodeling factor.

Authors:  T Nakagawa; M Bulger; M Muramatsu; T Ito
Journal:  J Biol Chem       Date:  2001-05-01       Impact factor: 5.157
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  23 in total

1.  Single-particle tracking for DNA tether length monitoring.

Authors:  Noëlle Pouget; Cynthia Dennis; Catherine Turlan; Mikhail Grigoriev; Michaël Chandler; Laurence Salomé
Journal:  Nucleic Acids Res       Date:  2004-05-20       Impact factor: 16.971

2.  Twisting and untwisting a single DNA molecule covered by RecA protein.

Authors:  Renaud Fulconis; Aurélien Bancaud; Jean-Francois Allemand; Vincent Croquette; Marie Dutreix; Jean-Louis Viovy
Journal:  Biophys J       Date:  2004-10       Impact factor: 4.033

3.  Quantitative guidelines for force calibration through spectral analysis of magnetic tweezers data.

Authors:  Aartjan J W te Velthuis; Jacob W J Kerssemakers; Jan Lipfert; Nynke H Dekker
Journal:  Biophys J       Date:  2010-08-09       Impact factor: 4.033

4.  Fast, long-range, reversible conformational fluctuations in nucleosomes revealed by single-pair fluorescence resonance energy transfer.

Authors:  Miroslav Tomschik; Haocheng Zheng; Ken van Holde; Jordanka Zlatanova; Sanford H Leuba
Journal:  Proc Natl Acad Sci U S A       Date:  2005-02-22       Impact factor: 11.205

5.  Compaction kinetics on single DNAs: purified nucleosome reconstitution systems versus crude extract.

Authors:  Gaudeline Wagner; Aurélien Bancaud; Jean-Pierre Quivy; Cédric Clapier; Geneviève Almouzni; Jean-Louis Viovy
Journal:  Biophys J       Date:  2005-08-12       Impact factor: 4.033

6.  Thin-foil magnetic force system for high-numerical-aperture microscopy.

Authors:  J K Fisher; J Cribb; K V Desai; L Vicci; B Wilde; K Keller; R M Taylor; J Haase; K Bloom; E Timothy O'Brien; R Superfine
Journal:  Rev Sci Instrum       Date:  2006-02       Impact factor: 1.523

7.  Micromanipulation studies of chromatin fibers in Xenopus egg extracts reveal ATP-dependent chromatin assembly dynamics.

Authors:  Jie Yan; Thomas J Maresca; Dunja Skoko; Christian D Adams; Botao Xiao; Morten O Christensen; Rebecca Heald; John F Marko
Journal:  Mol Biol Cell       Date:  2006-11-15       Impact factor: 4.138

8.  Homebuilt single-molecule scanning confocal fluorescence microscope studies of single DNA/protein interactions.

Authors:  Haocheng Zheng; Lori S Goldner; Sanford H Leuba
Journal:  Methods       Date:  2007-03       Impact factor: 3.608

9.  Pulling the chromatin.

Authors:  C Claudet; J Bednar
Journal:  Eur Phys J E Soft Matter       Date:  2006-03-14       Impact factor: 1.890

10.  Quantitative detection of small molecule/DNA complexes employing a force-based and label-free DNA-microarray.

Authors:  Dominik Ho; Christian Dose; Christian H Albrecht; Philip Severin; Katja Falter; Peter B Dervan; Hermann E Gaub
Journal:  Biophys J       Date:  2009-06-03       Impact factor: 4.033
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