Literature DB >> 787929

Integration of synthetic globin genes into an E. coli plasmid.

K O Wood, J C Lee.   

Abstract

Rabbit globin mRNA has been purified and used as a template by reverse transcriptase. The resulting duplex molecule consisting of rabbit globin mRNA/cNDA has been linked in vitro to Eco RI cleaved plasmid Col E1 DNA. Transformation of E. coli C6OO by this recombinant molecule has been achieved. Transformed bacteria acquire the colicin EQ immunity of Col E1 and a closed circular DNA species of 4.40-4.45 x 10 (6) daltons in molecular weight, an increase of 2.0-2.5 x 10(5) daltons compared to that of the parent plasmid DNA. In addition , 3H cDNA synthesized from globin RNA hybridized perferentially to the recombinant plasmid DNA.

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Year:  1976        PMID： 787929      PMCID： PMC343052          DOI： 10.1093/nar/3.8.1961

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  24 in total

1.  Molecular weights of separated rabbit alpha-and beta-globin messenger RNAs.

Authors:  J J Shearman; P H Hamlyn; H J Gould
Journal:  FEBS Lett       Date:  1974-10-01       Impact factor: 4.124

2.  A versatile composite gel column for the electrophoretic separation of ribonucleic acid molecules.

Authors:  H E Savage; R J Kuchler
Journal:  Prep Biochem       Date:  1971

3.  Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA.

Authors:  T H Rabbitts
Journal:  Nature       Date:  1976-03-18       Impact factor: 49.962

4.  Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.

Authors:  F Rougeon; P Kourilsky; B Mach
Journal:  Nucleic Acids Res       Date:  1975-12       Impact factor: 16.971

5.  Enzymatic in vitro synthesis of globin genes.

Authors:  A Efstratiadis; F C Kafatos; A M Maxam; T Maniatis
Journal:  Cell       Date:  1976-02       Impact factor: 41.582

6.  Full length and discrete partial reverse transcripts of globin and chorion mRNAs.

Authors:  A Efstratiadis; T Maniatis; F C Kafatos; A Jeffrey; J N Vournakis
Journal:  Cell       Date:  1975-04       Impact factor: 41.582

7.  Mechanism of action of ribonucleic acid-directed deoxyribonucleic acid polymerase. I. Transcription of globin messenger ribonucleic acid.

Authors:  A K Falvey; J A Kantor; M G Robert-Guroff; D J Picciano; G B Weiss; J M Vavich; W F Anderson
Journal:  J Biol Chem       Date:  1974-11-25       Impact factor: 5.157

8.  Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli.

Authors:  D A Jackson; R H Symons; P Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1972-10       Impact factor: 11.205

9.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

10.  Replication and transcription of eukaryotic DNA in Escherichia coli.

Authors:  J F Morrow; S N Cohen; A C Chang; H W Boyer; H M Goodman; R B Helling
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205
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  11 in total

1.  Recombinant DNA clones constructed from immunoglobulin kappa light chain messenger RNA.

Authors:  R Wall; M Gilmore-Hebert; R Higuchi; M Komaromy; G Paddock; J Strommer; W Salser
Journal:  Nucleic Acids Res       Date:  1978-09       Impact factor: 16.971

2.  Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin structural gene.

Authors:  P Humphries; M Cochet; A Krust; P Gerlinger; P Kourilsky; P Chambon
Journal:  Nucleic Acids Res       Date:  1977-07       Impact factor: 16.971

3.  High-efficiency cloning of full-length cDNA.

Authors:  H Okayama; P Berg
Journal:  Mol Cell Biol       Date:  1982-02       Impact factor: 4.272

4.  Structure of recombinant plasmids containing synthetic human foetal globin gene sequences.

Authors:  P Humphries; L W Coggins; R W Old; G J Mitchell; C Coleclough; J Paul
Journal:  Mol Gen Genet       Date:  1978-09-20

5.  5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.

Authors:  H Land; M Grez; H Hauser; W Lindenmaier; G Schütz
Journal:  Nucleic Acids Res       Date:  1981-05-25       Impact factor: 16.971

6.  mRNA abundance changes during flagellar regeneration in Chlamydomonas reinhardtii.

Authors:  J A Schloss; C D Silflow; J L Rosenbaum
Journal:  Mol Cell Biol       Date:  1984-03       Impact factor: 4.272

7.  Alcohol dehydrogenase in Drosophila: isolation and characterization of messenger RNA and cDNA clone.

Authors:  C Benyajati; N Wang; A Reddy; E Weinberg; W Sofer
Journal:  Nucleic Acids Res       Date:  1980-12-11       Impact factor: 16.971

8.  Molecular cloning of the genome of poliovirus type 1.

Authors:  S van der Werf; F Bregegere; H Kopecka; N Kitamura; P G Rothberg; P Kourilsky; E Wimmer; M Girard
Journal:  Proc Natl Acad Sci U S A       Date:  1981-10       Impact factor: 11.205

9.  cDNA cloning of a human autoimmune nuclear ribonucleoprotein antigen.

Authors:  E D Wieben; A M Rohleder; J M Nenninger; T Pederson
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

10.  Escherichia coli susceptible to glycopeptide antibiotics.

Authors:  D M Shlaes; J H Shlaes; J Davies; R Williamson
Journal:  Antimicrob Agents Chemother       Date:  1989-02       Impact factor: 5.191
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