Literature DB >> 802513

Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.

F Rougeon, P Kourilsky, B Mach.   

Abstract

Double stranded DNA has been synthesized in vitro from rabbit globin messenger RNA and elongated with homopolymeric dG tails. An E. coli plasmid was cleaved by EcoRI. The cohesive ends were repaired and dC tails added, to permit reconstitution of the EcoRI sites upon annealing with the dG elongated globin DNA. Transformation of E. coli with the globin-plasmid DNA hybrid has yielded a clone which harbours a recombinant plasmid (pCR1-betaG1), as demonstrated by hybridization experiments with radioactive globin cDNA. The sequence carried by the recombinant plasmid corresponds to part of the gene sequence coding for the beta chain of rabbit globin. Circular DNA of the purified recombinant plasmid exhibits sensitivity to EcoRI.

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Year:  1975        PMID： 802513      PMCID： PMC343603          DOI： 10.1093/nar/2.12.2365

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Electrophoresis of RNA in formamide.

Authors:  J C Pinder; D Z Staynov; W B Gratzer
Journal:  Biochemistry       Date:  1974-12-17       Impact factor: 3.162

2.  DNA nucleotide sequence restricted by the RI endonuclease.

Authors:  J Hedgpeth; H M Goodman; H W Boyer
Journal:  Proc Natl Acad Sci U S A       Date:  1972-11       Impact factor: 11.205

3.  Kinetic studies of gene frequency. I. Use of a DNA copy of reticulocyte 9 S RNA to estimate globin gene dosage in mouse tissues.

Authors:  P R Harrison; G D Birnie; A Hell; S Humphries; B D Young; J Paul
Journal:  J Mol Biol       Date:  1974-04-25       Impact factor: 5.469

4.  Enzymatic synthesis of DNA complementary to purified 14S messenger RNA of immunoglobulin light chain.

Authors:  H Digglemann; C H Faust; B Mach
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

5.  The separation and identification of alpha- and beta-globin messenger ribonucleic acids.

Authors:  M R Morrison; S A Brinkley; J Gorski; J B Lingrel
Journal:  J Biol Chem       Date:  1974-08-25       Impact factor: 5.157

6.  A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.

Authors:  P C Wensink; D J Finnegan; J E Donelson; D S Hogness
Journal:  Cell       Date:  1974-12       Impact factor: 41.582

7.  Proteolytic cleavage fo native DNA polymerase into two different catalytic fragments. Influence of assay condtions on the change of exonuclease activity and polymerase activity accompanying cleavage.

Authors:  H Klenow; K Overgaard-Hansen; S A Patkar
Journal:  Eur J Biochem       Date:  1971-10-14

8.  Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli.

Authors:  D A Jackson; R H Symons; P Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1972-10       Impact factor: 11.205

9.  Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.

Authors:  L Katz; D T Kingsbury; D R Helinski
Journal:  J Bacteriol       Date:  1973-05       Impact factor: 3.490

10.  Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.

Authors:  V Hershfield; H W Boyer; C Yanofsky; M A Lovett; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205
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  43 in total

1.  Sequence analysis of cloned cDNA encoding part of an immunoglobulin heavy chain.

Authors:  J Rogers; P Clarke; W Salser
Journal:  Nucleic Acids Res       Date:  1979-07-25       Impact factor: 16.971

2.  Cloning of immunoglobulin kappa light chain genes from mouse liver and myeloma MOPC 173.

Authors:  M Steinmetz; H G Zachau; B Mach
Journal:  Nucleic Acids Res       Date:  1979-07-25       Impact factor: 16.971

3.  Recombinant DNA clones constructed from immunoglobulin kappa light chain messenger RNA.

Authors:  R Wall; M Gilmore-Hebert; R Higuchi; M Komaromy; G Paddock; J Strommer; W Salser
Journal:  Nucleic Acids Res       Date:  1978-09       Impact factor: 16.971

4.  Isolation of native DNA fragments containing structural genes at the beginning, in the middle or at the end of the coding strand.

Authors:  G N Yenikolopov; A P Ryskov; T Nitta; G P Georgiev
Journal:  Nucleic Acids Res       Date:  1976-10       Impact factor: 16.971

5.  Genetic engineering and plasmids.

Authors:  B Mach
Journal:  Experientia       Date:  1977-01-15

6.  Correction of complex heteroduplexes made of mouse H-2 gene sequences in Escherichia coli K-12.

Authors:  B Cami; P Chambon; P Kourilsky
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

7.  5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.

Authors:  H Land; M Grez; H Hauser; W Lindenmaier; G Schütz
Journal:  Nucleic Acids Res       Date:  1981-05-25       Impact factor: 16.971

8.  Excision of DNA segments introduced into cloning vectors by the poly(dA-dT) joining method.

Authors:  S P Goff; P Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

9.  Recombinant plasmids containing Xenopus laevis globin structural genes derived from complementary DNA.

Authors:  P Humphries; R Old; L W Coggins; T McShane; C Watson; J Paul
Journal:  Nucleic Acids Res       Date:  1978-03       Impact factor: 16.971

10.  Integration of synthetic globin genes into an E. coli plasmid.

Authors:  K O Wood; J C Lee
Journal:  Nucleic Acids Res       Date:  1976-08       Impact factor: 16.971
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