| Literature DB >> 768776 |
Abstract
DNA copies of rabbit globin mRNA have been inserted into the plasmid mini-ColEl. After addition of poly(dT) tails, the plasmid was used to prime the copying of mRNA by reverse transcriptase. Mini-ColEl carrying globin sequences were denatured and annealed with mini-ColEl tailed with poly(dA). Functional plasmids containing the synthesised globin sequences were isolated by transfection in E. coli.Entities:
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Year: 1976 PMID: 768776 DOI: 10.1038/260221a0
Source DB: PubMed Journal: Nature ISSN: 0028-0836 Impact factor: 49.962