Literature DB >> 6780981

Alcohol dehydrogenase in Drosophila: isolation and characterization of messenger RNA and cDNA clone.

C Benyajati, N Wang, A Reddy, E Weinberg, W Sofer.   

Abstract

The mRNA for alcohol dehydrogenase (ADH) in D. melanogaster has been identified by translation in a cell-free system. The in vitro synthesized polypeptide, specifically precipitated by anti-ADH antibody, has identical subunit molecular weight (25,000 daltons) and tryptic peptide profile to the in vivo synthesized ADH. The poly A containing ADH-mRNA has been purified by specific precipitation of ADH-polysomes using anti-ADH antibody and S. aureus. Transformation of E. coli with the dA-tailed ADH-mRNA-complementary DNA hybrid annealed to the dT-tailed pBR322 yielded one plasmid which has been identified as the ADH-cDNA clone. The identification involved hybridization selection of ADH-mRNA and in vitro translation, in situ hybridization to the Adh locus on salivary gland polytene chromosomes and DNA sequencing. This ADH-cDNA plasmid contains 349 bases of the C-terminal protein coding and 180 bases of the 3' untranslated region.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 6780981      PMCID： PMC324332          DOI： 10.1093/nar/8.23.5649

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  35 in total

1.  The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for alcohol dehydrogenase. II. Lethal mutations in the region.

Authors:  R C Woodruff; M Ashburner
Journal:  Genetics       Date:  1979-05       Impact factor: 4.562

2.  Immunoprecipitation of specific polysomes using Staphylococcus aureus: purification of the immunoglobulin k chain messenger RNA from the mouse myeloma MPC11.

Authors:  N M Gough; J M Adams
Journal:  Biochemistry       Date:  1978-12-12       Impact factor: 3.162

3.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

4.  Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors:  D B Clewell
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

5.  Drosophila alcohol dehydrogenase. Purification and partial characterization.

Authors:  W Sofer; H Ursprung
Journal:  J Biol Chem       Date:  1968-06-10       Impact factor: 5.157

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Synthesis of full length cDNAs from four partially purified oviduct mRNAs.

Authors:  G N Buell; M P Wickens; F Payvar; R T Schimke
Journal:  J Biol Chem       Date:  1978-04-10       Impact factor: 5.157

8.  The syntheiss of high yields of full-length reverse transcripts of globin mRNA.

Authors:  E Y Friedman; M Rosbash
Journal:  Nucleic Acids Res       Date:  1977-10       Impact factor: 16.971

9.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

10.  Alcohol dehydrogenase-negative mutants in Drosophila: defects at the structural locus?

Authors:  M Schwartz; W Sofer
Journal:  Genetics       Date:  1976-05       Impact factor: 4.562
View more
  20 in total

1.  Chorion gene cis-regulatory DNA restricts tissue specificity of reporter gene expression in transformed Drosophila.

Authors:  B Bienz-Tadmor; P Tolias; B Stebbins-Boaz; B D Mariani; S A Gerbi; F C Kafatos
Journal:  Chromosoma       Date:  1992-08       Impact factor: 4.316

2.  Structural properties of double-stranded RNAs associated with biological control of chestnut blight fungus.

Authors:  J Tartaglia; C P Paul; D W Fulbright; D L Nuss
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

3.  The partial characterization of alcohol dehydrogenase null alleles from natural populations of Drosophila melanogaster.

Authors:  A L Freeth; J B Gibson; H G de Couet
Journal:  Biochem Genet       Date:  1986-12       Impact factor: 1.890

4.  Characterization of the AdhSL regulatory mutation in Drosophila melanogaster.

Authors:  D R Schott; P D East; K Paigen
Journal:  Genetics       Date:  1988-07       Impact factor: 4.562

5.  Molecular consequences of two formaldehyde-induced mutations in the alcohol dehydrogenase gene of Drosophila melanogaster.

Authors:  A R Place; C Benyajati; W Sofer
Journal:  Biochem Genet       Date:  1987-10       Impact factor: 1.890

6.  Adh expression in species of the mulleri subgroup of Drosophila.

Authors:  J Alberola; A Sanchez; A Fontdevila
Journal:  Biochem Genet       Date:  1987-10       Impact factor: 1.890

7.  Deletions at intervening sequence splice sites in the alcohol dehydrogenase gene of Drosophila.

Authors:  C Benyajati; A R Place; N Wang; E Pentz; W Sofer
Journal:  Nucleic Acids Res       Date:  1982-11-25       Impact factor: 16.971

8.  Cloned Drosophila alcohol dehydrogenase genes are correctly expressed after transfection into Drosophila cells in culture.

Authors:  C Benyajati; J F Dray
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

9.  Nucleotide sequence comparison of the Adh gene in three drosophilids.

Authors:  V H Cohn; M A Thompson; G P Moore
Journal:  J Mol Evol       Date:  1984       Impact factor: 2.395

10.  Biochemical and molecular analysis of naturally occurring Adh variants in Drosophila melanogaster.

Authors:  S M Anderson; J F McDonald
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.