Literature DB >> 100767

Recombinant DNA clones constructed from immunoglobulin kappa light chain messenger RNA.

R Wall, M Gilmore-Hebert, R Higuchi, M Komaromy, G Paddock, J Strommer, W Salser.   

Abstract

Recombinant DNA clones have been generated from mouse myeloma MOPC 21 immunoglobulin kappa light chain mRNA. Complementary DNA (cDNA) synthesized on kappa light chain mRNA by reverse transcriptase was made double stranded and inserted into the bacterial plasmid vector, pMB9. Approximately 70 tetracycline-resistant transformed colonies containing kappa light chain mRNA sequences were identified by colony hybridization. Five of these recombinant clones were selected and characterized. Three clones contain both kappa light chain constant and variable region sequences. Two of these three recombinant clones have been shown to include all of the kappa light chain constant and variable region coding sequences. Another of the five selected recombinant clones contain kappa light chain constant region sequences. The remaining characterized clone appears to be derived from sequences at the 5'-end of kappa light chain mRNA, possibly extending to the terminal cap structure.

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Year:  1978        PMID： 100767      PMCID： PMC342235          DOI： 10.1093/nar/5.9.3113

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  29 in total

1.  Reiteration frequency of immunoglobulin light chain genes: further evidence for somatic generation of antibody diversity.

Authors:  S Tonegawa
Journal:  Proc Natl Acad Sci U S A       Date:  1976-01       Impact factor: 11.205

2.  No detectable reiteration of genes coding for mouse MOPC 41 immunoglobulin light-chain mRNA.

Authors:  M G Farace; M F Aellen; P A Briand; C H Faust; P Vassalli; B Mach
Journal:  Proc Natl Acad Sci U S A       Date:  1976-03       Impact factor: 11.205

3.  Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA.

Authors:  T H Rabbitts
Journal:  Nature       Date:  1976-03-18       Impact factor: 49.962

4.  Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.

Authors:  F Rougeon; P Kourilsky; B Mach
Journal:  Nucleic Acids Res       Date:  1975-12       Impact factor: 16.971

5.  Characterization of light chain and light chain constant region fragment mRNAs in MPC 11 mouse myeloma cells and variants.

Authors:  W M Kuehl; B A Kaplan; M D Scharff
Journal:  Cell       Date:  1975-06       Impact factor: 41.582

6.  Determination of globin mRNA sequences and their insertion into bacterial plasmids.

Authors:  W Salser; F Browne; P Clarke; H Heindell; R Higuchi; G Paddock; J Roberts; G Studnicka; P Zakar
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1976

7.  Amplification and characterization of a beta-globin gene synthesized in vitro.

Authors:  T Maniatis; S G Kee; A Efstratiadis; F C Kafatos
Journal:  Cell       Date:  1976-06       Impact factor: 41.582

8.  Integration of synthetic globin genes into an E. coli plasmid.

Authors:  K O Wood; J C Lee
Journal:  Nucleic Acids Res       Date:  1976-08       Impact factor: 16.971

9.  Stepwise biosynthesis in vitro of globin genes from globin mRNA by DNA polymerase of avian myeloblastosis virus.

Authors:  F Rougeon; B Mach
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

10.  A general method for cloning eukaryotic structural gene sequences.

Authors:  R Higuchi; G V Paddock; R Wall; W Salser
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205
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  7 in total

1.  Sequence analysis of cloned cDNA encoding part of an immunoglobulin heavy chain.

Authors:  J Rogers; P Clarke; W Salser
Journal:  Nucleic Acids Res       Date:  1979-07-25       Impact factor: 16.971

2.  Variable and constant regions are separated in the 10-kbase transcription unit coding for immunoglobulin kappa light chains.

Authors:  M Gilmore-Herbert; K Hercules; M Komaromy; R Wall
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

3.  RNA metabolism in nuclei: selective transport of kappa exons from myeloma nuclei and adenoviral transcripts from infected HeLa nuclei.

Authors:  R Patterson; E Werner; J Fetherston
Journal:  Nucleic Acids Res       Date:  1986-05-27       Impact factor: 16.971

4.  Mutant immunoglobulin genes have repetitive DNA elements inserted into their intervening sequences.

Authors:  R G Hawley; M J Shulman; H Murialdo; D M Gibson; N Hozumi
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

5.  Functional immunoglobulin M production after transfection of cloned immunoglobulin heavy and light chain genes into lymphoid cells.

Authors:  A Ochi; R G Hawley; T Hawley; M J Shulman; A Traunecker; G Köhler; N Hozumi
Journal:  Proc Natl Acad Sci U S A       Date:  1983-10       Impact factor: 11.205

6.  RNA metabolism in isolated nuclei: processing and transport of immunoglobulin light chain sequences.

Authors:  C Otegui; R J Patterson
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971

7.  Mouse immunoglobulin D: construction and characterization of a cloned delta chain cDNA.

Authors:  J F Mushinski; F R Blattner; J D Owens; F D Finkelman; S W Kessler; L Fitzmaurice; M Potter; P W Tucker
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205
  7 in total

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