| Literature DB >> 4600264 |
J F Morrow, S N Cohen, A C Chang, H W Boyer, H M Goodman, R B Helling.
Abstract
Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated from E. coli minicells harboring the plasmids hybridizes to amplified X. laevis rDNA.Entities:
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Year: 1974 PMID: 4600264 PMCID: PMC388315 DOI: 10.1073/pnas.71.5.1743
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205