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Literature DB >> 7826325

The effect of low temperatures on enzyme activity.

Abstract

The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain AN1 and beta-glucosidase from Caldocellum saccharolyticum expressed in Escherichia coli) has been exploited to allow measurement of activity over a 175 degrees C temperature range, from +90 degrees C to -85 degrees C for the glutamate dehydrogenase and from +90 degrees C to -70 degrees C for the beta-glucosidase. The Arrhenius plots of these enzymes, and those for two mesophilic enzymes (glutamate dehydrogenase from bovine liver and beta-galactosidase from Escherichia coli), exhibit no downward deflection corresponding to the glass transition, found by biophysical measurements of several non-enzymic mesophilic proteins at about -65 degrees C and reflecting a sharp decrease in protein flexibility as the overall motion of groups of atoms ceases.

Entities: Gene Species

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Year: 1995 PMID： 7826325 PMCID： PMC1136422 DOI： 10.1042/bj3050017

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

19 in total

1. The beta-galactosidase-catalyzed hydrolysis of o-nitrophenol-beta-D-galactoside at subzero temperatures: evidence for a galactosyl-enzyme intermediate.

Authors: A L Fink; K J Angelides
Journal: Biochem Biophys Res Commun Date: 1975-05-19 Impact factor: 3.575

Review 2. Enzymology at subzero temperatures.

Authors: P Douzou
Journal: Adv Enzymol Relat Areas Mol Biol Date: 1977

3. Correlation between the amide proton exchange rates and the denaturation temperatures in globular proteins related to the basic pancreatic trypsin inhibitor.

Authors: G Wagner; K Wüthrich
Journal: J Mol Biol Date: 1979-05-05 Impact factor: 5.469

4. Protein states and proteinquakes.

Authors: A Ansari; J Berendzen; S F Bowne; H Frauenfelder; I E Iben; T B Sauke; E Shyamsunder; R D Young
Journal: Proc Natl Acad Sci U S A Date: 1985-08 Impact factor: 11.205

5. Glutamate dehydrogenase from the extremely thermophilic archaebacterial isolate AN1.

Authors: R C Hudson; L D Ruttersmith; R M Daniel
Journal: Biochim Biophys Acta Date: 1993-10-06

6. Conformational substates in a protein: structure and dynamics of metmyoglobin at 80 K.

Authors: H Hartmann; F Parak; W Steigemann; G A Petsko; D R Ponzi; H Frauenfelder
Journal: Proc Natl Acad Sci U S A Date: 1982-08 Impact factor: 11.205

7. Evidence for a correlation between the photoinduced electron transfer and dynamic properties of the chromatophore membranes from Rhodospirillum rubrum.

Authors: F Parak; E N Frolov; A A Kononenko; R L Mössbauer; V I Goldanskii; A B Rubin
Journal: FEBS Lett Date: 1980-08-11 Impact factor: 4.124

8. Protein dynamics. Mössbauer spectroscopy on deoxymyoglobin crystals.

Authors: F Parak; E W Knapp; D Kucheida
Journal: J Mol Biol Date: 1982-10-15 Impact factor: 5.469

9. Dynamics of heme iron in crystals of metmyoglobin and deoxymyoglobin.

Authors: E R Bauminger; S G Cohen; I Nowik; S Ofer; J Yariv
Journal: Proc Natl Acad Sci U S A Date: 1983-02 Impact factor: 11.205

10. Stability and substrate specificity of a beta-glucosidase from the thermophilic bacterium Tp8 cloned into Escherichia coli.

Authors: A R Plant; J E Oliver; M L Patchett; R M Daniel; H W Morgan
Journal: Arch Biochem Biophys Date: 1988-04 Impact factor: 4.013

15 in total

9. Enzyme-dependent fluorescence recovery of NADH after photobleaching to assess dehydrogenase activity of isolated perfused hearts.

Authors: Angel Moreno; Sarah Kuzmiak-Glancy; Rafael Jaimes; Matthew W Kay
Journal: Sci Rep Date: 2017-03-31 Impact factor: 4.379

10. Mg2+ decreases arrhenius energies of activation for high temperature catalysis of phosphatases in Thermoactinomyces vulgaris.

Authors: Ved Pal Singh
Journal: Curr Microbiol Date: 2007-07-25 Impact factor: 2.343

The effect of low temperatures on enzyme activity.

1. The beta-galactosidase-catalyzed hydrolysis of o-nitrophenol-beta-D-galactoside at subzero temperatures: evidence for a galactosyl-enzyme intermediate.

Review 2. Enzymology at subzero temperatures.

3. Correlation between the amide proton exchange rates and the denaturation temperatures in globular proteins related to the basic pancreatic trypsin inhibitor.

4. Protein states and proteinquakes.

5. Glutamate dehydrogenase from the extremely thermophilic archaebacterial isolate AN1.

6. Conformational substates in a protein: structure and dynamics of metmyoglobin at 80 K.

7. Evidence for a correlation between the photoinduced electron transfer and dynamic properties of the chromatophore membranes from Rhodospirillum rubrum.

8. Protein dynamics. Mössbauer spectroscopy on deoxymyoglobin crystals.

9. Dynamics of heme iron in crystals of metmyoglobin and deoxymyoglobin.

10. Stability and substrate specificity of a beta-glucosidase from the thermophilic bacterium Tp8 cloned into Escherichia coli.

1. Enzyme activity and dynamics: xylanase activity in the absence of fast anharmonic dynamics.

2. Enzymatic conformational fluctuations along the reaction coordinate of cytidine deaminase.

3. Cryoenzymology of the hammerhead ribozyme.

4. Enzyme activity below the dynamical transition at 220 K.

Review 5. The denaturation and degradation of stable enzymes at high temperatures.

6. Activity and dynamics of an enzyme, pig liver esterase, in near-anhydrous conditions.

7. Enzyme dynamics and activity: time-scale dependence of dynamical transitions in glutamate dehydrogenase solution.

8. Establishment of Cutoff Values for Newborn Screening of Six Lysosomal Storage Disorders by Tandem Mass Spectrometry.

9. Enzyme-dependent fluorescence recovery of NADH after photobleaching to assess dehydrogenase activity of isolated perfused hearts.

10. Mg2+ decreases arrhenius energies of activation for high temperature catalysis of phosphatases in Thermoactinomyces vulgaris.