| Literature DB >> 7667088 |
K Tang1, D Fu, S Kötter, R J Cotter, C R Cantor, H Köster.
Abstract
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxypicolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.Entities:
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Year: 1995 PMID: 7667088 PMCID: PMC307169 DOI: 10.1093/nar/23.16.3126
Source DB: PubMed Journal: Nucleic Acids Res ISSN: 0305-1048 Impact factor: 16.971