Literature DB >> 9016613

Sequencing double-stranded DNA by strand displacement.

D J Fu1, H Köster, C L Smith, C R Cantor.   

Abstract

Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of dideoxynucleotides. This allows dsDNA targets to be captured from mixtures and directly sequenced without subcloning, purification or denaturation.

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Year:  1997        PMID: 9016613      PMCID: PMC146469          DOI: 10.1093/nar/25.3.677

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  8 in total

1.  A strategy for rapid and efficient DNA sequencing by mass spectrometry.

Authors:  H Köster; K Tang; D J Fu; A Braun; D van den Boom; C L Smith; R J Cotter; C R Cantor
Journal:  Nat Biotechnol       Date:  1996-09       Impact factor: 54.908

2.  Efficient preparation of short DNA sequence ladders potentially suitable for MALDI-TOF DNA sequencing.

Authors:  D J Fu; N E Broude; H Köster; C L Smith; C R Cantor
Journal:  Genet Anal       Date:  1996-01

Review 3.  Single-stranded DNA binding proteins required for DNA replication.

Authors:  J W Chase; K R Williams
Journal:  Annu Rev Biochem       Date:  1986       Impact factor: 23.643

4.  A DNA sequencing strategy that requires only five bases of known terminal sequence for priming.

Authors:  D J Fu; N E Broude; H Köster; C L Smith; C R Cantor
Journal:  Proc Natl Acad Sci U S A       Date:  1995-10-24       Impact factor: 11.205

5.  Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes.

Authors:  K Tang; D Fu; S Kötter; R J Cotter; C R Cantor; H Köster
Journal:  Nucleic Acids Res       Date:  1995-08-25       Impact factor: 16.971

6.  Enhanced DNA sequencing by hybridization.

Authors:  N E Broude; T Sano; C L Smith; C R Cantor
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-12       Impact factor: 11.205

7.  Multiplex strand displacement amplification (SDA) and detection of DNA sequences from Mycobacterium tuberculosis and other mycobacteria.

Authors:  G T Walker; J G Nadeau; P A Spears; J L Schram; C M Nycz; D D Shank
Journal:  Nucleic Acids Res       Date:  1994-07-11       Impact factor: 16.971

8.  Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.

Authors:  N D Sinha; J Biernat; J McManus; H Köster
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

  8 in total
  1 in total

1.  Linear nicking endonuclease-mediated strand-displacement DNA amplification.

Authors:  Aric Joneja; Xiaohua Huang
Journal:  Anal Biochem       Date:  2011-02-20       Impact factor: 3.365

  1 in total

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