| Literature DB >> 9016613 |
D J Fu1, H Köster, C L Smith, C R Cantor.
Abstract
Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of dideoxynucleotides. This allows dsDNA targets to be captured from mixtures and directly sequenced without subcloning, purification or denaturation.Entities:
Mesh:
Year: 1997 PMID: 9016613 PMCID: PMC146469 DOI: 10.1093/nar/25.3.677
Source DB: PubMed Journal: Nucleic Acids Res ISSN: 0305-1048 Impact factor: 16.971