Literature DB >> 282602

Chemical synthesis of genes for human insulin.

R Crea, A Kraszewski, T Hirose, K Itakura.   

Abstract

A rapid chemical procedure has been developed and used for the synthesis of 29 oligodeoxyribonucleotides to build synthetic genes for human insulin. The gene for insulin B chain, 104 base pairs, and the one for A chain, 77 base pairs, were designed from the amino acid sequence of human polypeptides. They bear single-stranded cohesive termini for the EcoRI and BamHI restriction endonucleases and are designed to be inserted separately into a pBR322 plasmid. The synthetic fragments, deca- to pentadecanucleotides, were synthesized by a block phosphotriester method with trinucleotides as building blocks. Final purification was by high-performance liquid chromatography. All 29 oligonucleotides were pure and had the correct sequences.

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Year:  1978        PMID： 282602      PMCID： PMC393054          DOI： 10.1073/pnas.75.12.5765

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  10 in total

1.  Resynthesis of insulin from its glycyl and phenylalanyl chains.

Authors:  Y C DU; Y S ZHANG; Z X LU; C L TSOU
Journal:  Sci Sin       Date:  1961-05

2.  Synthetic lac operator DNA is functional in vivo.

Authors:  H L Heyneker; J Shine; H M Goodman; H W Boyer; J Rosenberg; R E Dickerson; S A Narang; K Itakura; S Lin; A D Riggs
Journal:  Nature       Date:  1976-10-28       Impact factor: 49.962

3.  Improved triester approach for the synthesis of pentadecathymidylic acid.

Authors:  K Itakura; N Katagiri; C P Bahl; R H Wightman; S A Narang
Journal:  J Am Chem Soc       Date:  1975-12-10       Impact factor: 15.419

4.  Chemical synthesis and sequence studies of deoxyribooligonucleotides which constitute the duplex sequence of the lactose operator of Escherichia coli.

Authors:  K Itakura; N Katagiri; S A Narang; C P Bahl; K J Marians; R Wu
Journal:  J Biol Chem       Date:  1975-06-25       Impact factor: 5.157

5.  Sequence analysis of synthetic oligonucleotides by high-performance liquid anion-exchange chromatography.

Authors:  J H van Boom; J F de Rooy
Journal:  J Chromatogr       Date:  1977-01-21

Review 6.  [Synthesis of insulin: beginning and progress].

Authors:  K Lübke; H Klostermeyer
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1970

7.  Chemical synthesis of restriction enzyme recognition sites useful for cloning.

Authors:  R H Scheller; R E Dickerson; H W Boyer; A D Riggs; K Itakura
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

8.  DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors:  E Jay; R Bambara; R Padmanabhan; R Wu
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971

9.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

10.  Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.

Authors:  K Itakura; T Hirose; R Crea; A D Riggs; H L Heyneker; F Bolivar; H W Boyer
Journal:  Science       Date:  1977-12-09       Impact factor: 47.728
  10 in total
  50 in total

1.  Regions of poliovirus protein VP1 produced in Escherichia coli induce neutralizing antibodies.

Authors:  M E Hoatlin; O M Kew; M E Renz
Journal:  J Virol       Date:  1987-05       Impact factor: 5.103

2.  A comprehensive list of chemically synthesized genes.

Authors:  G Gröger; F Ramalho-Ortigao; H Steil; H Seliger
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

3.  Penicillin acylase from E. coli: unique gene-protein relation.

Authors:  G Schumacher; D Sizmann; H Haug; P Buckel; A Böck
Journal:  Nucleic Acids Res       Date:  1986-07-25       Impact factor: 16.971

4.  A new life in a bacterium through synthetic genome: a successful venture by craig venter.

Authors:  Shailly Anand; Jaya Malhotra; Ankita Dua; Nidhi Garg; Anjali Saxena; Naseer Sangwan; Devi Lal; Mansi Verma; Swati Jindal; Jaspreet Kaur; Kirti Kumari; Aeshna Nigam; Neha Niharika; Jasvinder Kaur; Rup Lal
Journal:  Indian J Microbiol       Date:  2010-09-16       Impact factor: 2.461

5.  Synthetic DNA and medicine.

Authors:  A D Riggs; K Itakura
Journal:  Am J Hum Genet       Date:  1979-09       Impact factor: 11.025

6.  Cloning, characterization, and expression of two alpha-amylase genes from Aspergillus niger var. awamori.

Authors:  D R Korman; F T Bayliss; C C Barnett; C L Carmona; K H Kodama; T J Royer; S A Thompson; M Ward; L J Wilson; R M Berka
Journal:  Curr Genet       Date:  1990-03       Impact factor: 3.886

7.  The primary structure of the Saccharomyces cerevisiae gene for 3-phosphoglycerate kinase.

Authors:  R A Hitzeman; F E Hagie; J S Hayflick; C Y Chen; P H Seeburg; R Derynck
Journal:  Nucleic Acids Res       Date:  1982-12-11       Impact factor: 16.971

8.  Plasmid-directed synthesis of hepatitis B surface antigen in monkey cells.

Authors:  C W Crowley; C C Liu; A D Levinson
Journal:  Mol Cell Biol       Date:  1983-01       Impact factor: 4.272

9.  Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.

Authors:  V Kohli; A Balland; M Wintzerith; R Sauerwald; A Staub; J P Lecocq
Journal:  Nucleic Acids Res       Date:  1982-11-25       Impact factor: 16.971

10.  A system for shotgun DNA sequencing.

Authors:  J Messing; R Crea; P H Seeburg
Journal:  Nucleic Acids Res       Date:  1981-01-24       Impact factor: 16.971
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