Literature DB >> 6897677

Molecular cloning and characterization of a complete DNA copy of potato spindle tuber viroid RNA.

P van Wezenbeek, P Vos, J van Boom, A van Kammen.   

Abstract

Double-stranded cDNA has been synthesized from RNA of a severe strain of potato spindle tuber viroid using a synthetic oligodeoxyribonucleotide as a primer. Upon cloning in bacteriophage M13mp9, two recombinant phages were selected to construct a pBR322-derived plasmid containing a complete viroid DNA copy. Elucidation of the nucleotide sequence revealed four differences with the previously established sequence of another PSTV strain, three of which were base exchanges and one a deletion.

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Year:  1982        PMID： 6897677      PMCID： PMC327061          DOI： 10.1093/nar/10.24.7947

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Citrus exocortis viroid: nucleotide sequence and secondary structure of an Australian isolate.

Authors:  J E Visvader; A R Gould; G E Bruening; R H Symons
Journal:  FEBS Lett       Date:  1982-01-25       Impact factor: 4.124

2.  Sensitive and rapid diagnosis of potato spindle tuber viroid disease by nucleic Acid hybridization.

Authors:  R A Owens; T O Diener
Journal:  Science       Date:  1981-08-07       Impact factor: 47.728

3.  A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.

Authors:  G N Godson; D Vapnek
Journal:  Biochim Biophys Acta       Date:  1973-04-11

4.  Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.

Authors:  A Panet; J H van de Sande; P C Loewen; H G Khorana; A J Raae; J R Lillehaug; K Kleppe
Journal:  Biochemistry       Date:  1973-12-04       Impact factor: 3.162

5.  Cloned poliovirus complementary DNA is infectious in mammalian cells.

Authors:  V R Racaniello; D Baltimore
Journal:  Science       Date:  1981-11-20       Impact factor: 47.728

6.  A system for shotgun DNA sequencing.

Authors:  J Messing; R Crea; P H Seeburg
Journal:  Nucleic Acids Res       Date:  1981-01-24       Impact factor: 16.971

7.  Chrysanthemum stunt viroid: primary sequence and secondary structure.

Authors:  J Haseloff; R H Symons
Journal:  Nucleic Acids Res       Date:  1981-06-25       Impact factor: 16.971

8.  Molecular mechanisms of nucleotide-sequence rearrangements in cDNA clones of human fibroblast interferon mRNA.

Authors:  G Volckaert; J Tavernier; R Derynck; R Devos; W Fiers
Journal:  Gene       Date:  1981-11       Impact factor: 3.688

9.  Avocado sunblotch viroid: primary sequence and proposed secondary structure.

Authors:  R H Symons
Journal:  Nucleic Acids Res       Date:  1981-12-11       Impact factor: 16.971

10.  A severe and a mild potato spindle tuber viroid isolate differ in three nucleotide exchanges only.

Authors:  H J Gross; U Liebl; H Alberty; G Krupp; H Domdey; K Ramm; H L Sänger
Journal:  Biosci Rep       Date:  1981-03       Impact factor: 3.840
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  11 in total

1.  Generation of viroid conformational isomers that are stable to incubation with magnesium ions and in a nuclear extract from tomato plants.

Authors:  U Pace; A D Branch; H D Robertson
Journal:  Nucleic Acids Res       Date:  1992-12-25       Impact factor: 16.971

2.  Compilation and analysis of viroid and viroid-like RNA sequences.

Authors:  F Bussière; D Lafontaine; J P Perreault
Journal:  Nucleic Acids Res       Date:  1996-05-15       Impact factor: 16.971

3.  Use of intramolecular chimeras to map molecular determinants of symptom severity of potato spindle tuber viroid (PSTVd).

Authors:  A Góra; T Candresse; W Zagórski
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

4.  Analysis of the population structure of three phenotypically different PSTVd isolates.

Authors:  A Góra; T Candresse; W Zagórski
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

5.  Primary and secondary structure of a 360-nucleotide isolate of potato spindle tuber viroid.

Authors:  D K Lakshman; S M Tavantzis
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

6.  Structure of viroid replicative intermediates: physico-chemical studies on SP6 transcripts of cloned oligomeric potato spindle tuber viroid.

Authors:  G Steger; M Tabler; W Brüggemann; M Colpan; G Klotz; H L Sänger; D Riesner
Journal:  Nucleic Acids Res       Date:  1986-12-22       Impact factor: 16.971

7.  Construction of infectious potato spindle tuber viroid cDNA clones.

Authors:  D E Cress; M C Kiefer; R A Owens
Journal:  Nucleic Acids Res       Date:  1983-10-11       Impact factor: 16.971

8.  Complexes of viroids with histones and other proteins.

Authors:  P Wolff; R Gilz; J Schumacher; D Riesner
Journal:  Nucleic Acids Res       Date:  1985-01-25       Impact factor: 16.971

9.  Correlation between structure and pathogenicity of potato spindle tuber viroid (PSTV).

Authors:  M Schnölzer; B Haas; K Raam; H Hofmann; H L Sänger
Journal:  EMBO J       Date:  1985-09       Impact factor: 11.598

10.  Primary structure and gene organization of the middle-component RNA of cowpea mosaic virus.

Authors:  P van Wezenbeek; J Verver; J Harmsen; P Vos; A van Kammen
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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