Literature DB >> 17847478

Sensitive and rapid diagnosis of potato spindle tuber viroid disease by nucleic Acid hybridization.

R A Owens, T O Diener.   

Abstract

A sensitive and reliable new method for the detection of potato spindle tuber viroid in potato tubers has been developed. The method is based on hybridization of highly radioactive recombinant DNA to viroid RNA that has been attached to a solid support. The method can be automated and permits the rapid testing of large numbers of tubers.

Entities:  

Year:  1981        PMID： 17847478     DOI： 10.1126/science.213.4508.670

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  18 in total

1.  Construction of a multiprobe for the simultaneous detection of viroids infecting citrus trees.

Authors:  Oded Cohen; Ozgur Batuman; Gulshan Stanbekova; Teruo Sano; Munir Mawassi; Moshe Bar-Joseph
Journal:  Virus Genes       Date:  2006-12       Impact factor: 2.332

2.  A rapid screening technique for the detection of repeated DNA sequences in plant tissues.

Authors:  J Hutchinson; A Abbott; M O Dell; R B Flavell
Journal:  Theor Appl Genet       Date:  1985-05       Impact factor: 5.699

3.  An improved procedure for the rapid one-step-cloning of full-length viroid cDNA.

Authors:  H Puchta; H L Sänger
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

4.  Biotinylated nucleic acid hybridization probes for potato virus detection.

Authors:  H E Hopp; L Giavedoni; M A Mandel; A Arese; B Orman; F Bravo Almonacid; H N Torres; A N Mentaberry
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

5.  Detection of enteroviruses by spot hybridization.

Authors:  T Hyypiä; P Stålhandske; R Vainionpää; U Pettersson
Journal:  J Clin Microbiol       Date:  1984-03       Impact factor: 5.948

6.  Studies towards the development of chemically synthesized non-radioactive biotinylated nucleic acid hybridization probes.

Authors:  A H Al-Hakim; R Hull
Journal:  Nucleic Acids Res       Date:  1986-12-22       Impact factor: 16.971

7.  Detection of avocado sunblotch viroid (ASBV) by dot-spot self-hybridization with a [(32)P]-labelled ASBV-RNA.

Authors:  A Rosner; S Spiegel; M Alper; M Bar-Joseph
Journal:  Plant Mol Biol       Date:  1983-01       Impact factor: 4.076

8.  The molecular structure of hop latent viroid (HLV), a new viroid occurring worldwide in hops.

Authors:  H Puchta; K Ramm; H L Sänger
Journal:  Nucleic Acids Res       Date:  1988-05-25       Impact factor: 16.971

9.  Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy.

Authors:  T Ohno; N Takamatsu; T Meshi; Y Okada
Journal:  Nucleic Acids Res       Date:  1983-09-24       Impact factor: 16.971

10.  Construction of infectious potato spindle tuber viroid cDNA clones.

Authors:  D E Cress; M C Kiefer; R A Owens
Journal:  Nucleic Acids Res       Date:  1983-10-11       Impact factor: 16.971
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